LOCUS       sequence-160271-        4335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway donor vector for TIR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-TIR1
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2460..2559)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2576..2595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2577..2595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2600..2616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2600..2616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2718..2737)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2729..3538
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2796..2815)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3685..4273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4174..4193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc aaaatgcaga agcgaatagc cttgtcgttt ccagaagagg tactagagca
      721 tgtgttctcg tttattcagc tggataagga taggaactca gtctctctgg tgtgcaagtc
      781 atggtacgag atcgagcggt ggtgcaggag gaaagtcttc atcgggaact gctacgccgt
      841 gagtccagcg acggtgatta ggaggttccc gaaagtgaga tccgtggagc ttaaaggaaa
      901 acctcacttt gctgacttta atttggtacc tgacggatgg ggaggttacg tgtatccatg
      961 gattgaggcc atgtcttcgt cttacacgtg gcttgaagag ataaggctga agaggatggt
     1021 ggtcaccgac gattgcttgg agctcatagc caagtctttt aagaatttta aggttcttgt
     1081 gctttcttcc tgcgaaggct tctccaccga tggtctcgct gctatcgctg ccacttgcag
     1141 gaatctgaaa gagcttgact tacgagagag tgatgttgac gacgttagtg gccactggct
     1201 tagccatttc ccagatacat acacttcttt ggtatcactc aatatatctt gcttagcatc
     1261 tgaggtcagt ttctctgctc tggaaaggct ggtgactagg tgtcccaatc tcaagtctct
     1321 caagcttaac cgagctgttc cacttgaaaa attggctact ttacttcaaa gagcacctca
     1381 attggaggaa ttgggcactg gtgggtacac tgcagaagtg cgaccagatg tttactctgg
     1441 tttatctgta gcgctctctg ggtgcaagga attgaggtgc ttatctggat tttgggatgc
     1501 tgttcctgcc tatcttccag cagtttattc ggtttgcagt cggcttacaa ctttgaatct
     1561 gagttatgca acagtccaga gctatgatct tgtcaagctt ctttgtcaat gccctaaact
     1621 gcagcgcctc tgggtgcttg actacatcga ggatgctggt cttgaggtgc ttgcttcaac
     1681 ctgcaaggac ctacgcgagc tgagagtgtt tccgtccgag ccttttgtca tggaaccaaa
     1741 tgtggcattg acggaacagg ggcttgtctc cgtctccatg ggctgtccaa aactcgagtc
     1801 ggttctctac ttctgccgtc aaatgaccaa tgctgcattg ataaccattg ctaggaaccg
     1861 tcccaacatg actcgcttcc gtttgtgcat cattgagcca aaagccccag actatctgac
     1921 tctagagcca ctggatattg gatttggagc catagtagag cactgcaagg atctccgtcg
     1981 cctctctcta tctggcctct tgaccgacaa ggtttttgaa tacattggga catatgccaa
     2041 gaagatggaa atgctctcag tggcatttgc aggagacagt gacttaggca tgcatcatgt
     2101 tttgtccggg tgcgatagct tgaggaaact agagataagg gactgcccgt ttggagacaa
     2161 ggcgcttttg gccaatgctt caaagctgga gacaatgcga tccctttgga tgtcttcttg
     2221 ttccgtgagt tttggagcct gcaagttact aggacagaag atgccaaagc tgaatgtgga
     2281 agtcatcgat gaacggggtg caccggactc gagaccagag agctgccctg ttgagagagt
     2341 cttcatatac cgaacagtgg ctggtcctcg atttgacatg cctggcttcg tctggaacat
     2401 ggaccaagac tcaacaatga ggttttccag gcaaatcatt actactaacg gattataaca
     2461 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2521 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     2581 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2641 tctctgatgt tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc
     2701 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     2761 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     2821 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     2881 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     2941 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     3001 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     3061 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     3121 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     3181 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     3241 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     3301 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     3361 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     3421 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     3481 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     3541 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     3601 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3661 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3721 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3781 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3841 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3901 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3961 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4021 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4081 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4141 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4201 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4261 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4321 ttttgctcac atgtt
//
