LOCUS       sequence-160313-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CA_WT
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatgcctcc acgaccatca tcaggtgaac
      121 tgtggggcat ccacttgatg cccccaagaa tcctagtaga atgtttacta ccaaatggaa
      181 tgatagtgac tttagaatgc ctccgtgagg ctacattaat aaccataaag catgaactat
      241 ttaaagaagc aagaaaatac cccctccatc aacttcttca agatgaatct tcttacattt
      301 tcgtaagtgt tactcaagaa gcagaaaggg aagaattttt tgatgaaaca agacgacttt
      361 gtgaccttcg gctttttcaa ccctttttaa aagtaattga accagtaggc aaccgtgaag
      421 aaaagatcct caatcgagaa attggttttg ctatcggcat gccagtgtgt gaatttgata
      481 tggttaaaga tccagaagta caggacttcc gaagaaatat tctgaacgtt tgtaaagaag
      541 ctgtggatct tagggacctc aattcacctc atagtagagc aatgtatgtc tatcctccaa
      601 atgtagaatc ttcaccagaa ttgccaaagc acatatataa taaattagat aaagggcaaa
      661 taatagtggt gatctgggta atagtttctc caaataatga caagcagaag tatactctga
      721 aaatcaacca tgactgtgta ccagaacaag taattgctga agcaatcagg aaaaaaactc
      781 gaagtatgtt gctatcctct gaacaactaa aactctgtgt tttagaatat cagggcaagt
      841 atattttaaa agtgtgtgga tgtgatgaat acttcctaga aaaatatcct ctgagtcagt
      901 ataagtatat aagaagctgt ataatgcttg ggaggatgcc caatttgatg ttgatggcta
      961 aagaaagcct ttattctcaa ctgccaatgg actgttttac
//
