LOCUS       sequence-160378-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing KRAS , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KRAS_WT
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             93..659
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(661..760)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(777..796)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(778..796)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(801..817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(801..817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatgactga atataaactt gtggtagttg
      121 gagctggtgg cgtaggcaag agtgccttga cgatacagct aattcagaat cattttgtgg
      181 acgaatatga tccaacaata gaggattcct acaggaagca agtagtaatt gatggagaaa
      241 cctgtctctt ggatattctc gacacagcag gtcaagagga gtacagtgca atgagggacc
      301 agtacatgag gactggggag ggctttcttt gtgtatttgc cataaataat actaaatcat
      361 ttgaagatat tcaccattat agagaacaaa ttaaaagagt taaggactct gaagatgtac
      421 ctatggtcct agtaggaaat aaatgtgatt tgccttctag aacagtagac acaaaacagg
      481 ctcaggactt agcaagaagt tatggaattc cttttattga aacatcagca aagacaagac
      541 agggtgttga tgatgccttc tatacattag ttcgagaaat tcgaaaacat aaagaaaaga
      601 tgagcaaaga tggtaaaaag aagaaaaaga agtcaaagac aaagtgtgta attatgtaat
      661 tgccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
      721 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
      781 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
      841 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
      901 aggacagaaa tgcctcgact tcgctgct
//
