LOCUS       sequence-160406-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LexA-GAD under the control of 57F03 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Yapici N, Cohn R, Schusterreiter C, Ruta V, Vosshall LB
  TITLE     A Taste Circuit that Regulates Ingestion by Integrating Food and 
            Hunger Signals.
  JOURNAL   Cell. 2016 Apr 21;165(3):715-29. doi: 10.1016/j.cell.2016.02.061. 
            Epub 2016 Mar 31.
  PUBMED    27040496
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.061"; journalName: "Cell"; date: 
            "2016-04-21- 21"; volume: "165"; issue: "3"; pages: "715-29"
COMMENT     Sequence Label: 57F03_LexA-GAD
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             140..160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(170..421,422..775)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 ggggtggctg agagcatcag ttgtgaatga atgttcgagc cgagcagacg tgccgctgcc
       61 ttcgttaata tcctttgaat aagccaactt tgaatcacaa gacgcatacc aaacggtacc
      121 gctagcaatc aaaatgccac ccaagaagaa gcgaaaagta gaagatccaa tgaaggctct
      181 cacggcccga caacaggaag tttttgattt gatacgggat catatatccc aaacgggtat
      241 gcctccgacc cgcgcagaga tagcacagcg actgggcttt cgatcgccta acgccgcgga
      301 ggagcacttg aaggcactgg cccgcaaggg tgtcattgaa atcgtgtccg gtgcgagccg
      361 cggaatccgg ctgttgcagg aggaggaaga gggcctgcca ctggtgggac gcgtggccgc
      421 tggcgagccg ctgctggccc agcaacacat agagggacac tatcaggtgg acccctcctt
      481 gtttaagcca aatgctgatt tcctgttgcg ggtgtcggga atgtccatga aggacatcgg
      541 tattatggat ggtgacctcc tcgccgtcca taagacacag gatgtcagga acggccaggt
      601 agtcgttgcc aggatagacg atgaggtcac tgtgaaacgt ctcaagaagc aaggcaataa
      661 ggtcgagctg ctgccggaga atagcgagtt caagccgatc gtggtggatc tgcgacagca
      721 gtcctttact atcgagggct tggccgtggg tgtgatccgc aacggagatt ggctgggatc
      781 catcgacagt gcggcccacc acgacaacag caccattcca ctggacttca tgcctcgtga
      841 cgcactgcat ggatttgact ggagcgagga ggatgatatg agcgacggac tcccattcct
      901 gaagaccgac cccaataaca atggtttctt tggcgacggc agcctgctgt gtatcctgcg
      961 aagtattggt tttaagccgg aaaattacac gaacagtaat gtcaatcgtc ttcccacaat
     1021 gatcaccgac cgttacacgc tggccagtcg cagtactaca agccgcctgc tgcaaagcta
     1081 tcttaacaac ttccacccct
//
