LOCUS       sequence-160524-         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To introduce dCas9-fused Tet1-CD and sgRNA2.0 system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu GL, Hu 
            R
  TITLE     A CRISPR-based approach for targeted DNA demethylation.
  JOURNAL   Cell Discov. 2016 May 3;2:16009. doi: 10.1038/celldisc.2016.9. 
            eCollection 2016.
  PUBMED    27462456
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-05-3"; volume: "2:16009. doi"; pages: " 
            10.1038/celldisc.2016.9. eCollection 2016"
COMMENT     Sequence Label: pdCas9-Tet1-CD
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        64..82
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        134..152
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        195..480
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 atatatcttg tggaaaggac gaaacaccgg gtcttcgaga agacctgttt tagagctagg
       61 ccaacatgag gatcacccat gtctgcaggg cctagcaagt taaaataagg ctagtccgtt
      121 atcaacttgg ccaacatgag gatcacccat gtctgcaggg ccaagtggca ccgagtcggt
      181 gcttttttgg tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      241 gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg
      301 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      361 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg
      421 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      481 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
      541 attttgtatt
//
