LOCUS       sequence-160884-       14207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ABA-inducible VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14207)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 14207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40P...
FEATURES             Location/Qualifiers
     source          1..14207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..284
                     /label=chicken beta-actin promoter
     intron          286..1303
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1227..1249
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1311..1330
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1358..1367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1940..2089
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             2096..2116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2546..2917
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             2936..3505
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_feature    join(3523..4096,4097..4099,4100..4108)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3690..3707)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3917..3936
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4124..4498
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    4536..5124
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4589..4609)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5007..5018)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5163..5182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5217..5236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5278..5777
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5313..5336)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5710..5729
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6737..7435
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             7457..7477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10658..10705
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     repeat_region   10895..10957
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11008..11028)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11008..11026)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11047..11063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11047..11063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11060..11082)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11071..11087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11095..11101,11102..11119,11120..11125))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11140..11161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11278..11295)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11449..12037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11529..11548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12208..12999,13000..13068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12831..12850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13069..13173)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13199..13654)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13336..13357)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13548..13567
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13781..13803
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13795..13812
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13796..13812
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13822..13841
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13822..13840
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   13870..13904
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
       61 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      121 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      181 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      241 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg
      301 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      361 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      421 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
      481 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
      541 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
      601 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
      661 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
      721 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
      781 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
      841 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
      901 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
      961 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     1021 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     1081 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     1141 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     1201 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     1261 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1321 ggttattgtg ctgtctcatc attttggcaa agaattcgcc accatgggtg ggggcgcgcc
     1381 aactcaagac gaattcaccc aactctccca atcaatcgcc gagttccaca cgtaccaact
     1441 cggtaacggc cgttgctcat ctctcctagc tcagcgaatc cacgcgccgc cggaaacagt
     1501 atggtccgtg gtgagacgtt tcgataggcc acagatttac aaacacttca tcaaaagctg
     1561 taacgtgagt gaagatttcg agatgcgagt gggatgcacg cgcgacgtga acgtgataag
     1621 tggattaccg gcgaatacgt ctcgggagag attagatctg ttggacgatg atcggagagt
     1681 gactgggttt agtataaccg gtggtgaaca taggctgagg aattataaat cggttacgac
     1741 ggttcataga tttgagaaag aagaagaaga agaaaggatc tggaccgttg ttttggaatc
     1801 ttatgttgtt gatgtaccgg aaggtaattc ggaggaagat acgagattgt ttgctgatac
     1861 ggttattaga ttgaatcttc agaaacttgc ttcgatcact gaagctatga acgaattctc
     1921 aagcgctgca ggtactagtg atgctttaga cgattttgac ttagatatgc ttggttcaga
     1981 cgcgttagac gacttcgacc tagacatgtt aggctcagat gcattggacg acttcgattt
     2041 agatatgttg ggctccgatg ccctagatga ctttgatcta gatatgctag gtagtcccaa
     2101 aaagaagagg aaagtgggat cccagtatct gcccgacaca gatgatagac accgaatcga
     2161 agagaaacgc aagcgaacgt atgaaacctt caaatcgatc atgaagaaat cgcccttctc
     2221 gggtccgacc gatcccaggc ccccaccgag aaggattgcg gtcccgtccc gctcgtcggc
     2281 cagcgtgccg aagcctgcgc cgcagcccta ccccttcacg tcgagcctga gcacaatcaa
     2341 ttatgacgag ttcccgacga tggtgttccc ctcgggacaa atctcacaag cctcggcgct
     2401 cgcaccagcg cctccccaag tccttccgca agcgcctgcc ccagcgcctg caccggcaat
     2461 ggtgtccgcc ctcgcacagg cccctgcgcc cgtccccgtg ctcgcgcctg gaccgcccca
     2521 ggcggtcgct ccaccggctc cgaagccgac gcaggccgga gagggaacac tctccgaagc
     2581 acttcttcaa ctccagtttg atgacgagga tcttggagca ctccttggaa actcgacaga
     2641 ccctgcggtg tttaccgacc tcgcgtcagt agataactcc gaatttcagc agcttttgaa
     2701 ccagggtatc ccggtcgcgc cacatacaac ggagcccatg ttgatggaat accccgaagc
     2761 aatcacgaga cttgtgacgg gagcgcagcg gcctcccgat cccgcacccg cacctttggg
     2821 ggcacctggc ctccctaacg gacttttgag cggcgacgag gatttctcct ccatcgccga
     2881 tatggatttc tcagccttgc tgtcacagat ttccagcggc tctggcagcg gcagccggga
     2941 ttccagggaa gggatgtttt tgccgaagcc tgaggccggc tccgctatta gtgacgtgtt
     3001 tgagggccgc gaggtgtgcc agccaaaacg aatccggcca tttcatcctc caggaagtcc
     3061 atgggccaac cgcccactcc ccgccagcct cgcaccaaca ccaaccggtc cagtacatga
     3121 gccagtcggg tcactgaccc cggcaccagt ccctcagcca ctggatccag cgcccgcagt
     3181 gactcccgag gccagtcacc tgttggagga tcccgatgaa gagacgagcc aggctgtcaa
     3241 agcccttcgg gagatggccg atactgtgat tccccagaag gaagaggctg caatctgtgg
     3301 ccaaatggac ctttcccatc cgcccccaag gggccatctg gatgagctga caaccacact
     3361 tgagtccatg accgaggatc tgaacctgga ctcacccctg accccggaat tgaacgagat
     3421 tctggatacc ttcctgaacg acgagtgcct cttgcatgcc atgcatatca gcacaggact
     3481 gtccatcttc gacacatctc tgttttgaat ttaaataatt cgcccctctc cctccccccc
     3541 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     3601 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     3661 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     3721 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     3781 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     3841 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     3901 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     3961 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacgtgtgt
     4021 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     4081 aaaacacgat gataatatgg ccacaaccgg atccggagct accatggcca agttgaccag
     4141 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     4201 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     4261 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     4321 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     4381 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     4441 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgagt
     4501 ttaaaccatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
     4561 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4621 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4681 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4741 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4801 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4861 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4921 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4981 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5041 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5101 tctccctttg ggccgcctcc ccgcatcctt gtttattgca gcttataatg gttacaaata
     5161 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5221 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ctgcaggtaa ttctaccggg
     5281 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5341 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     5401 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     5461 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     5521 tcactaggct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     5581 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     5641 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     5701 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     5761 catctccggg cctttcgacc tgcagcccaa gcttgtcgac gccaccatga cgcgtgtgcc
     5821 tttgtatggt tttacttcga tttgtggaag aagacctgag atggaagctg ctgtttcgac
     5881 tataccaaga ttccttcaat cttcctctgg ttcgatgtta gatggtcggt ttgatcctca
     5941 atccgccgct catttcttcg gtgtttacga cggccatggc ggttctcagg tagcgaacta
     6001 ttgtagagag aggatgcatt tggctttggc ggaggagata gctaaggaga aaccgatgct
     6061 ctgcgatggt gatacgtggc tggagaagtg gaagaaagct cttttcaact cgttcctgag
     6121 agttgactcg gagattgagt cagttgcgcc ggagacggtt gggtcaacgt cggtggttgc
     6181 cgttgttttc ccgtctcaca tcttcgtcgc taactgcggt gactctagag ccgttctttg
     6241 ccgcggcaaa actgcacttc cattatccgt tgaccataaa ccggatagag aagatgaagc
     6301 tgcgaggatt gaagccgcag gagggaaagt gattcagtgg aatggagctc gtgttttcgg
     6361 tgttctcgcc atgtcgagat ccattggcga tagatacttg aaaccatcca tcattcctga
     6421 tccggaagtg acggctgtga agagagtaaa agaagatgat tgtctgattt tggcgagtga
     6481 cggggtttgg gatgtaatga cggatgaaga agcgtgtgag atggcaagga agcggattct
     6541 cttgtggcac aagaaaaacg cggtggctgg ggatgcatcg ttgctcgcgg atgagcggag
     6601 aaaggaaggg aaagatcctg cggcgatgtc cgcggctgag tatttgtcaa agctggcgat
     6661 acagagagga agcaaagaca acataagtgt ggtggtggtt gatttgaagc atatgtcaag
     6721 cgctgcaggt gctaccatga gcgagctgat taaggagaac atgcacatga agctgtacat
     6781 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta
     6841 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     6901 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg
     6961 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     7021 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     7081 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa
     7141 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga
     7201 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa
     7261 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt
     7321 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga
     7381 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc ttaatggtgg
     7441 aggctctgga atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
     7501 caagcggaac tacatcctgg gcctggccat cggcatcacc agcgtgggct acggcatcat
     7561 cgactacgag acacgggacg tgatcgatgc cggcgtgcgg ctgttcaaag aggccaacgt
     7621 ggaaaacaac gagggcaggc ggagcaagag aggcgccaga aggctgaagc ggcggaggcg
     7681 gcatagaatc cagagagtga agaagctgct gttcgactac aacctgctga ccgaccacag
     7741 cgagctgagc ggcatcaacc cctacgaggc cagagtgaag ggcctgagcc agaagctgag
     7801 cgaggaagag ttctctgccg ccctgctgca cctggccaag agaagaggcg tgcacaacgt
     7861 gaacgaggtg gaagaggaca ccggcaacga gctgtccacc aaagagcaga tcagccggaa
     7921 cagcaaggcc ctggaagaga aatacgtggc cgaactgcag ctggaacggc tgaagaaaga
     7981 cggcgaagtg cggggcagca tcaacagatt caagaccagc gactacgtga aagaagccaa
     8041 acagctgctg aaggtgcaga aggcctacca ccagctggac cagagcttca tcgacaccta
     8101 catcgacctg ctggaaaccc ggcggaccta ctatgaggga cctggcgagg gcagcccctt
     8161 cggctggaag gacatcaaag aatggtacga gatgctgatg ggccactgca cctacttccc
     8221 cgaggaactg cggagcgtga agtacgccta caacgccgac ctgtacaacg ccctgaacga
     8281 cctgaacaat ctcgtgatca ccagggacga gaacgagaag ctggaatatt acgagaagtt
     8341 ccagatcatc gagaacgtgt tcaagcagaa gaagaagccc accctgaagc agatcgccaa
     8401 agaaatcctc gtgaacgaag aggatattaa gggctacaga gtgaccagca ccggcaagcc
     8461 cgagttcacc aacctgaagg tgtaccacga catcaaggac attaccgccc ggaaagagat
     8521 tattgagaac gccgagctgc tggatcagat tgccaagatc ctgaccatct accagagcag
     8581 cgaggacatc caggaagaac tgaccaatct gaactccgag ctgacccagg aagagatcga
     8641 gcagatctct aatctgaagg gctataccgg cacccacaac ctgagcctga aggccatcaa
     8701 cctgatcctg gacgagctgt ggcacaccaa cgacaaccag atcgctatct tcaaccggct
     8761 gaagctggtg cccaagaagg tggacctgtc ccagcagaaa gagatcccca ccaccctggt
     8821 ggacgacttc atcctgagcc ccgtcgtgaa gagaagcttc atccagagca tcaaagtgat
     8881 caacgccatc atcaagaagt acggcctgcc caacgacatc attatcgagc tggcccgcga
     8941 gaagaactcc aaggacgccc agaaaatgat caacgagatg cagaagcgga accggcagac
     9001 caacgagcgg atcgaggaaa tcatccggac caccggcaaa gagaacgcca agtacctgat
     9061 cgagaagatc aagctgcacg acatgcagga aggcaagtgc ctgtacagcc tggaagccat
     9121 ccctctggaa gatctgctga acaacccctt caactatgag gtggaccaca tcatccccag
     9181 aagcgtgtcc ttcgacaaca gcttcaacaa caaggtgctc gtgaagcagg aagaagccag
     9241 caagaagggc aaccggaccc cattccagta cctgagcagc agcgacagca agatcagcta
     9301 cgaaaccttc aagaagcaca tcctgaatct ggccaagggc aagggcagaa tcagcaagac
     9361 caagaaagag tatctgctgg aagaacggga catcaacagg ttctccgtgc agaaagactt
     9421 catcaaccgg aacctggtgg ataccagata cgccaccaga ggcctgatga acctgctgcg
     9481 gagctacttc agagtgaaca acctggacgt gaaagtgaag tccatcaatg gcggcttcac
     9541 cagctttctg cggcggaagt ggaagtttaa gaaagagcgg aacaaggggt acaagcacca
     9601 cgccgaggac gccctgatca ttgccaacgc cgatttcatc ttcaaagagt ggaagaaact
     9661 ggacaaggcc aaaaaagtga tggaaaacca gatgttcgag gaaaagcagg ccgagagcat
     9721 gcccgagatc gaaaccgagc aggagtacaa agagatcttc atcacccccc accagatcaa
     9781 gcacattaag gacttcaagg actacaagta cagccaccgg gtggacaaga agcctaatag
     9841 agagctgatt aacgacaccc tgtactccac ccggaaggac gacaagggca acaccctgat
     9901 cgtgaacaat ctgaacggcc tgtacgacaa ggacaatgac aagctgaaaa agctgatcaa
     9961 caagagcccc gaaaagctgc tgatgtacca ccacgacccc cagacctacc agaaactgaa
    10021 gctgattatg gaacagtacg gcgacgagaa gaatcccctg tacaagtact acgaggaaac
    10081 cgggaactac ctgaccaagt actccaaaaa ggacaacggc cccgtgatca agaagattaa
    10141 gtattacggc aacaaactga acgcccatct ggacatcacc gacgactacc ccaacagcag
    10201 aaacaaggtc gtgaagctgt ccctgaagcc ctacagattc gacgtgtacc tggacaatgg
    10261 cgtgtacaag ttcgtgaccg tgaagaatct ggatgtgatc aaaaaagaaa actactacga
    10321 agtgaatagc aagtgctatg aggaagctaa gaagctgaag aagatcagca accaggccga
    10381 gtttatcgcc tccttctaca acaacgatct gatcaagatc aacggcgagc tgtatagagt
    10441 gatcggcgtg aacaacgacc tgctgaaccg gatcgaagtg aacatgatcg acatcaccta
    10501 ccgcgagtac ctggaaaaca tgaacgacaa gaggcccccc aggatcatta agacaatcgc
    10561 ctccaagacc cagagcatta agaagtacag cacagacatt ctgggcaacc tgtatgaagt
    10621 gaaatctaag aagcaccctc agatcatcaa aaagggcaaa aggccggcgg ccacgaaaaa
    10681 ggccggccag gcaaaaaaga aaaagtaact cgagctagtt aaaagttttg ttactttata
    10741 gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg
    10801 ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta
    10861 ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat
    10921 ctttaacgta cgtcacaata tgattatctt tctagggtta atctagtata cgcgtatgcg
    10981 gccgcttaat taatccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
    11041 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    11101 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    11161 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    11221 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    11281 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11341 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11401 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11461 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11521 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11581 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11641 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11701 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11761 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11821 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11881 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11941 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    12001 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    12061 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    12121 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    12181 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    12241 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    12301 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    12361 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    12421 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    12481 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    12541 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    12601 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    12661 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    12721 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12781 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12841 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12901 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12961 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    13021 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    13081 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    13141 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
    13201 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    13261 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    13321 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    13381 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
    13441 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    13501 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    13561 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    13621 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
    13681 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    13741 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    13801 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg gcgcgccatt
    13861 ctagattaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
    13921 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
    13981 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
    14041 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
    14101 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
    14161 tttcttgtta tagatatcaa ctagaatgct agcatgggcc cagatct
//
