LOCUS       sequence-160975-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human mutant PC5A 
            (R511C) with C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX304 PCSK5 (R511C)-V5 blast
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..38
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actgtcggga tcaacaagtt tgtacaaaaa agttggcatg ggctggggga gccgctgctg
       61 ctgcccggga cgtttggacc tgctgtgcgt gctggcgctg ctcgggggct gcctgctccc
      121 cgtgtgtcgg acgcgcgtct acaccaacca ctgggcagtc aaaatcgccg ggggcttccc
      181 ggaggccaac cgtatcgcca gcaagtacgg attcatcaac ataggacaga taggggccct
      241 gaaggactac taccacttct accatagcag gacgattaaa aggtcagtta tctcgagcag
      301 agggacccac agtttcattt caatggaacc aaaggtggaa tggatccaac agcaagtggt
      361 aaaaaagcgg acaaagaggg attatgactt cagtcgtgcc cagtctacct atttcaatga
      421 tcccaagtgg cccagcatgt ggtatatgca ctgcagtgac aatacacatc cctgccagtc
      481 tgacatgaat atcgaaggag cctggaagag aggctacacg ggaaagaaca ttgtggtcac
      541 tatcctggat gacggaattg agagaaccca tccagatctg atgcaaaact acgatgctct
      601 ggcaagttgc gacgtgaatg ggaatgactt ggacccaatg cctcgttatg atgcaagcaa
      661 cgagaacaag catgggactc gctgtgctgg agaagtggca gccgctgcaa acaattcgca
      721 ctgcacagtc ggaattgctt tcaacgccaa gatcggagga gtgcgaatgc tggacggaga
      781 tgtcacggac atg
//
