LOCUS       sequence-160989-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for inducible expression of human SMAD4 with 
            N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pEN_TT 3xFLAG SMAD4
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..86
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cgtcagatcg ccactagatg gactacaaag accatgacgg tgattataaa gatcatgaca
       61 tcgattacaa ggatgacgat gacaagacta gtatggacaa tatgtctatt acgaatacac
      121 caacaagtaa tgatgcctgt ctgagcattg tgcatagttt gatgtgccat agacaaggtg
      181 gagagagtga aacatttgca aaaagagcaa ttgaaagttt ggtaaagaag ctgaaggaga
      241 aaaaagatga attggattct ttaataacag ctataactac aaatggagct catcctagta
      301 aatgtgttac catacagaga acattggatg ggaggcttca ggtggctggt cggaaaggat
      361 ttcctcatgt gatctatgcc cgtctctgga ggtggcctga tcttcacaaa aatgaactaa
      421 aacatgttaa atattgtcag tatgcgtttg acttaaaatg tgatagtgtc tgtgtgaatc
      481 catatcacta cgaacgagtt gtatcacctg gaattgatct ctcaggatta acactgcaga
      541 gtaatgctcc atcaagtatg atggtgaagg atgaatatgt gcatgacttt gagggacagc
      601 catcgttgtc cactgaagga cattcaattc aaaccatcca gcatccacca agtaatcgtg
      661 catcgacaga gacatacagc accccagctc tgttagcccc atctgagtct aatgctacca
      721 gcactgccaa ctttcccaac attcctgtgg cttccacaag tcagcctgcc agtatactgg
      781 g
//
