LOCUS       sequence-160990-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for inducible expression of human SMAD4 with 
            N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pEN_TT 3xFLAG SMAD4
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..186
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(225..337)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
       61 cacgggccag agctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct
      121 gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag
      181 ctgggtctag atatctcgaa aacgcattag tcttccaatt gaaaaaagtg atttaattta
      241 taccatttta attcagcttt gtaaaaatgt atcaaagaga tagcaaggta ttcagtttta
      301 gtaaacaaga taattgctcc taaagtagcc ccttgaattc tcagtctaaa ggttgtgggt
      361 ctgcaatcgg catggtatga agtacttcgt ctaggagctg gagggcccgg tgtaagtgaa
      421 tttcaatcca gcaaggtgtt tctttgatgc tctgtcttgg gtaatccggt ccccagcctt
      481 tcacaaaact catcctgagt atgcataagc gacgaaggtc atcaacacca attccagcag
      541 cagctgacag actgatagct ggagctattc cacctactga tcctgggcca gggatgtttc
      601 ctgccacggc tgctgcctgg gcagctgctg cagcttgtgc agtagccgcc tgctgctgca
      661 tctgtcgatg acactgacgc aaatcaaaga cctttatata tgcacttggg tagatcttat
      721 gaacagcatc tccaggtgca cgcccagctt ctctgtctaa gtagtaactc tgtacaa
//
