LOCUS       sequence-161077-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1a - Step1 vector a (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1a
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  127..351
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(485..502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
       61 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgtcacaa
      121 cgccgcgaat atagtcttta ccatgccctt ggccacgccc ctctttaata cgacgggcaa
      181 tttgcacttc agaaaatgaa gagtttgctt tagccataac aaaagtccag tatgcttttt
      241 cacagcataa ctggactgat ttcagtttac aactattctg tctagtttaa gactttattg
      301 tcatagttta gatctatttt gttcagttta agactttatt gtccgcccac acccgcttac
      361 gcagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc ggctggtcta
      421 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
      481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      661 ataggctccg cccccctgac gagcatcaca aaaatcgacg c
//
