LOCUS       sequence-161102-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH. 
            Hygromycin B resistance is encoded by the Mygro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-eGFP-IRESMygro
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgatgat aatatggcca caaccatggc gcgtatgaaa aagcccgagc tgactgccac
      181 ctctgtcgag aagttcctga ttgaaaagtt tgattccgtg agtgacctga tgcagctgag
      241 cgagggcgag gaatcccggg ccttctcttt tgacgtgggc ggaaggggct acgtgctgag
      301 agtcaactct tgcgccgacg gattctataa ggatcgctac gtgtatcgac actttgctag
      361 tgccgctctg cccatccctg aggtgctgga cattggcgag ttcagcgaat ccctgacata
      421 ctgtatctcc aggagagccc agggagtgac tctgcaggat ctgcctgaga ccgaactgcc
      481 tgccgtgctg cagcctgtgg ctgaagctat ggacgctatc gcagccgctg atctgtcaca
      541 gactagcggg ttcggcccat ttggccccca gggaattggg cagtacacca catggagaga
      601 ctttatctgc gcaattgccg atccccacgt gtatcattgg cagacagtca tggacgatac
      661 cgtgagcgcc agtgtcgctc aggcactgga cgagctgatg ctgtgggcag aggattgtcc
      721 tgaagtgagg cacctggtcc atgctgactt cgggagcaac aatgtgctga ccgataacgg
      781 cagaatcaca gccgtcattg actggagtga ggctatgttt ggcgattcac agtacgaagt
      841 ggctaatatc ttcttt
//
