LOCUS       sequence-161105-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH. 
            Hygromycin B resistance is encoded by the Mygro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-eGFP-IRESMygro
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..233
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(285..338)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
       61 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
      121 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      181 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      241 accaccccgg tgaacagctc ctcgcccttg ctcaccatac gcgccgggcc aggattctcc
      301 tccacgtcac cgcatgttag aagacttcct ctgccctctc ctccggaccc gccgccacgc
      361 gccattccct ccttggaggc catgtgggcc atgaggtcca ccaccctgtt gctgtagcca
      421 aattcgttgt catacctaga agatgaaaag agttgtcagg gccctttttc tgagccagcc
      481 accagagggc gccagaccct gcacttttta agagccagtc tctggcccca gccacatacc
      541 aggaaatgag cttgacaaag tggtcgttga gggcaatgcc agccccagcg tcaaaggtgg
      601 aggagtgggt gtcgctgttg aagtcagagg agaccacctg gtgctcagtg tagcccagga
      661 tgcccttgag ggggccctcc gacgcctgct tcaccacctt cttgatgtca tcatatttgg
      721 caggtttttc tagacggcag gtcaggtcca ccactgacac gttggcagtg gggacacgga
      781 aggccatgcc agtgagcttc ccgttcagct cagggatgac cttgcccaca gccttggcag
      841 cgccagtaga ggcagggatg atgttctgga gagccccgcg gccatcacgc cacagtttcc
      901 cggaggggcc atccacagtc ttctgggtgg cagtgatggc atg
//
