LOCUS       sequence-161150-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Middle entry vector containing GAL4 fused to ERT2 and the VP16 
            transcriptional activation domain for tamoxifen-inducible 
            UAS-promoted transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Akerberg AA, Stewart S, Stankunas K
  TITLE     Spatial and temporal control of transgene expression in zebrafish.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92217. doi: 
            10.1371/journal.pone.0092217. eCollection 2014.
  PUBMED    24643048
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92217. doi"; pages: " 
            10.1371/journal.pone.0092217. eCollection 2014"
COMMENT     Sequence Label: pME-GAL4-ERT2-VP16
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     139..159
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        141..159
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     196..212
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     196..212
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgatgagc aatgctttct tataatgcca actttgtaca agaaagctgg gtgctatgac
      121 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
      181 cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt cggcttgggg
      241 tcgacttacc caccgtactc gtcaattcca agggcatcgg taaacatctg ctcaaactcg
      301 aagtcggcca tatccagagc gccgtagggg gcggagtcgt ggggggtaaa tcccggaccc
      361 ggggaatccc cgtcccccaa catgtccaga tcgaaatcgt ctagcgcgtc ggcatgcgcc
      421 atcgccacgt cctcgccgtc taagtggagc tccgatgaag tagagcccgc agtggccaag
      481 tggctttggt ccgtctcctc cacggatgcc cctccacggc tagtgggcgc atgtaggcgg
      541 tgggcgtccg ccgcctcgag cagcaggtca tagaggggca ccacgttctt gcacttcatg
      601 ctgtacagat gctccatgcc tttgttactc atgtgcctga tgtgggagag gatgaggagg
      661 agctgggcca gccgctggtg ctgctgctgc agggtcaggc ctgccttggc catcaggtgg
      721 atcaaagtgt ctgtgatctt gtccaggact cggtggatat ggtccttctc ttccagagac
      781 ttcagggtgc tggacaga
//
