LOCUS       sequence-161159-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' entry vector containing frog translation elongation factor 1 
            alpha (EF1a) enhancer fused to rabbit b-globin intron for 
            semi-ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Akerberg AA, Stewart S, Stankunas K
  TITLE     Spatial and temporal control of transgene expression in zebrafish.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92217. doi: 
            10.1371/journal.pone.0092217. eCollection 2014.
  PUBMED    24643048
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92217. doi"; pages: " 
            10.1371/journal.pone.0092217. eCollection 2014"
COMMENT     Sequence Label: p5E-EF1a/b-globin
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..115
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     117..136
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        117..135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tacgctacca tggagctcca aataatgatt ttattttgac tgatagtgac ctgttcgttg
       61 caacaaattg ataagcaatg cttttttata atgccaactt tgtatagaaa agttggtaat
      121 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgag cagggggatc
      181 atctaatcaa gcacaaataa ggggcgtgta acacaaaagc cagcgaccct ttccaatgca
      241 aatcaaactt gcaattcttt gccgttttta tcatttaagt gtcggcttaa ggtccactat
      301 cagatgtaaa cagccttatc taacaaaggt atcattacat tctgaaattc tcaggcatgc
      361 aagctagctt atgacgcact agggagtgcc acccttcctt tcgccctaac ttcgtgataa
      421 ctcgcgcgtt tcactcaaca gctgcatccg ccctagtgct actgggagtt gtagtataca
      481 agacgcttac aggctgaatg ttctgtcaag accccgcctc tagcactttg ggaattctgg
      541 acttgatgat gtcatggtta atccccgccc agtagaggcg gctatataaa gggtggttaa
      601 ggcccggttc gctctcttcc tcaccgggtc tgcggcgagt tctagctgaa gcttcctgca
      661 ggtcgaccga tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
      721 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
      781 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcacttt
//
