LOCUS       sequence-161161-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' entry vector containing frog translation elongation factor 1 
            alpha (EF1a) enhancer fused to rabbit b-globin intron for 
            semi-ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Akerberg AA, Stewart S, Stankunas K
  TITLE     Spatial and temporal control of transgene expression in zebrafish.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92217. doi: 
            10.1371/journal.pone.0092217. eCollection 2014.
  PUBMED    24643048
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92217. doi"; pages: " 
            10.1371/journal.pone.0092217. eCollection 2014"
COMMENT     Sequence Label: p5E-EF1a/b-globin
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(68..191)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        192..210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     247..263
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     247..263
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcgcggccgc cctgcagctc tagagctcga attctacagg tcactaatac catctaagta
       61 gttggttcat agtgactgca tatgttgtgt tttacagtat tatgtagtct gttttttatg
      121 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttttt
      181 gtacaaactt gaattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc
      241 ggccgctcta gaactagtgg atccgtcgag gaattctttg ccaaaatgat gagacagcac
      301 aacaaccagc acgttgccca ggagctgtag gaaagagaag aaggcatgaa catggttagc
      361 agaggggccc ggtttggact cagagtattt tatcctcatc tcaaacagtg tatatcattg
      421 taaccataaa gagaaaggca ggatgatgac cagggtgtag ttgtttctac caataagaat
      481 atttccacgc cagccagaat ttatatgcag aaatattcta ccttatcatt taattataac
      541 aattgttctc taaaactgtg ctgaagtaca atataatata ccctgattgc cttgaaaaaa
      601 aagtgattag agaaagtact tacaatctga caaataaaca aaagtgaatt taaaaattcg
      661 ttacaaatgc aagctaaagt ttaacgaaaa agttacagaa aatgaaaaga aaataagagg
      721 agacaatggt tgtcaacaga gtagaaagtg aaagaaacaa aattatcatg agggtccatg
      781 gtgatacaag
//
