LOCUS       sequence-161166-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' entry vector containing the EF1a/B-globin fused to zebrafish 
            b-actin 2 enhancer/promoter for strong semi-ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-EF1a/b-actin
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..94
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     96..115
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        96..114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
       61 ttttttataa tgccaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat
      121 tgggtaccgg gccccccctc gaggtcgagc agggggatca tctaatcaag cacaaataag
      181 gggcgtgtaa cacaaaagcc agcgaccctt tccaatgcaa atcaaacttg caattctttg
      241 ccgtttttat catttaagtg tcggcttaag gtccactatc agatgtaaac agccttatct
      301 aacaaaggta tcattacatt ctgaaattct caggcatgca agctagctta tgacgcacta
      361 gggagtgcca cccttccttt cgccctaact tcgtgataac tcgcgcgttt cactcaacag
      421 ctgcatccgc cctagtgcta ctgggagttg tagtatacaa gacgcttaca ggctgaatgt
      481 tctgtcaaga ccccgcctct agcactttgg gaattctgga cttgatgatg tcatggttaa
      541 tccccgccca gtagaggcgg ctatataaag ggtggttaag gcccggttcg ctctcttcct
      601 caccgggtct gcggcgagtt ctagctgaag cttcctgcag gtcgacaaag caagtctctc
      661 acataatata tcttctaaaa gacagaaaac attactttca gaccatattg taatcaaatg
      721 aacactttca tattagtcaa taatattacg gaaatgtatt taaaaactga ataaacatat
      781 atttacacct ttgtctttat ttctagagtg ctt
//
