LOCUS       sequence-161175-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for integration at yeast Leu locus, expression 
            from the GPD promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     Nemhauser plasmids
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGP5G-ccdB
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..79
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    87..211
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(236..241,242..259,260..266)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(386..405)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttctactttt atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg
       61 attctagaac tagtggatcc cccatcacaa gtttgtacaa aaaagctgaa cgagaaacgt
      121 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
      181 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
      241 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
      301 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      361 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      421 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      481 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      541 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      601 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      661 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      721 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
      781 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccc
//
