LOCUS       sequence-161204-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of human ID2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shID2 #2
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(44..138)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    44..62
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(108..127)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(427..471)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acacggatat ctgcagatat ccgtgttgag ggtggtcccg gtgtctctat cactgatagg
       61 gaacttataa gtctctatca ctgataggga tttcacgttt atggtgattt cccagaacac
      121 atagcgacat gcaaatattg cggccgctcg aggctagtct cgtgatcgat accgtcgaga
      181 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
      241 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
      301 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
      361 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
      421 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
      481 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
      541 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
      601 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
      661 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
      721 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca a
//
