LOCUS       sequence-161209-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-HygMx4
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..270
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     terminator      352..371
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      complement(489..676)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     597..621
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(704..724)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atctttgaaa agataatgta tgattatgct ttcactcata tttatacaga aacttgatgt
       61 tttctttcga gtatatacaa ggtgattaca tgtacgtttg aagtacaact ctagattttg
      121 tagtgccctc ttgggctagc ggtaaaggtg cgcatttttt cacaccctac aatgttctgt
      181 tcaaaagatt ttggtcaaac gctgtagaag tgaaagttgg tgcgcatgtt tcggcgttcg
      241 aaacttctcc gcagtgaaag ataaatgatc agaatcgatg catttaaata gcaagttaaa
      301 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtggtg ctttttttgt
      361 tttttatgtc ttcgagtcat gtaattagtt ataagcatgt gagctaagac actgtaattg
      421 ccaatctaaa cgataccacg gccgctctag agaaatgggg agcgatttgc aggcatttgc
      481 tcggcatgcc ggtagaggtg tggtcaataa gagcgacctc atgctatacc tgagaaagca
      541 acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa acctaagagt
      601 cactttaaaa tttgtataca cttatttttt ttataactta tttaataata aaaatcataa
      661 atcataagaa attcgcctcg agagtctagg atccggaact acctaccttg cgctttttct
      721 tgggagctcc ctcatcccct ccgagctgtg agaggtcaat ccgagtttcg taaagtccgg
      781 tgatgctctg gtggatgagg gtggcgtcca gcacttcttt ggtggaggta tagcgtttcc
      841 ggtcgatggt ggtgtcgaag tacttg
//
