LOCUS       sequence-161237-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for integration at HIS3, expression from the ADH1
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Pierre-Jerome E, Jang SS, Havens KA, Nemhauser JL, Klavins E
  TITLE     Recapitulation of the forward nuclear auxin response pathway in 
            yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9407-12. doi: 
            10.1073/pnas.1324147111. Epub 2014 Jun 16.
  PUBMED    24979769
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1324147111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9407-12"
COMMENT     Sequence Label: pGP8A-ccdB
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     148..164
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     148..164
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    172..296
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(321..326,327..344,345..351)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(471..490)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc
       61 attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca
      121 agcatacaat caactatctc atatacatct agaactagtg gatcccccat cacaagtttg
      181 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
      241 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
      301 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
      361 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      421 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      481 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      541 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      601 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
      661 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      721 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
      781 gttacggtga aaacctggcc tatttcccta a
//
