LOCUS       sequence-161241-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for integration at HIS3, expression from the ADH1
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Pierre-Jerome E, Jang SS, Havens KA, Nemhauser JL, Klavins E
  TITLE     Recapitulation of the forward nuclear auxin response pathway in 
            yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9407-12. doi: 
            10.1073/pnas.1324147111. Epub 2014 Jun 16.
  PUBMED    24979769
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1324147111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9407-12"
COMMENT     Sequence Label: pGP8A-ccdB
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(118..242)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(275..291)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(276..292)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      294..540
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(310..328)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
       61 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat
      121 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
      181 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg
      241 gtgatgggct gcaggaattc gatttcaagc ttatcgatac cgtcgacctc gagtcatgta
      301 attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga
      361 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
      421 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
      481 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg
      541 tgacaccgat tatttaaagc tgcagcatac gatatatata catgtgtata tatgtatacc
      601 tatgaatgtc agtaagtatg tatacgaaca gtatgatact gaagatgaca aggtaatgca
      661 tcattctata cgtgtcattc tgaacgaggc gcgctttcct tttttctttt tgctttttct
      721 ttttttttct cttgaactcg agaaaaaaaa tataaaagag atggaggaac gggaaaaagt
      781 tagttgtggt gataggtggc aagtggtatt ccgtaagaac aac
//
