LOCUS       sequence-161279-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of human GDF11 
            (cross reacts with mouse Gdf11).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shGDF11 #1
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(56..150)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    56..74
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    84..102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(120..139)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(439..483)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acatcgcacc taagcgctac aactgcagtt gtagcgctta ggtgcgatgc cggtgtctct
       61 atcactgata gggaacttat aagtctctat cactgatagg gatttcacgt ttatggtgat
      121 ttcccagaac acatagcgac atgcaaatat tgcggccgct cgaggctagt ctcgtgatcg
      181 ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
      241 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
      301 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
      361 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
      421 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
      481 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
      541 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
      601 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
      661 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
      721 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctca
//
