LOCUS       sequence-161296-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of mouse Id2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shId2 #1
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(40..134)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(104..123)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(423..467)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    652..885
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aataaactgc agtttattta gccacagagt actccggtgt ctctatcact gatagggaac
       61 ttataagtct ctatcactga tagggatttc acgtttatgg tgatttccca gaacacatag
      121 cgacatgcaa atattgcggc cgctcgaggc tagtctcgtg atcgataccg tcgagatccg
      181 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
      241 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
      301 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
      361 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
      421 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
      481 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
      541 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
      601 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
      661 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
      721 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
      781 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
      841 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
      901 ttttctctct gcacnactct tctcttttgn cttggtgggt gctacnccta agggntcaan
      961 tttttactac cttaaaattt aaaaanattc actttcncca atngnccccc catatcnnnc
     1021 tcccnccagg tctgaaaaac aagcgggccg ccttngctgg ggcggggggg ccnnactttg
     1081 ggttnggccc ctccccnacc tnggccaaag
//
