LOCUS       sequence-161298-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing FRT-flanked kanamycin resistance cassette
            (KanR) with pA for FLP-induced kanamycin resistance, drug selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-FRT-KanR-FRT pA
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..127
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    190..324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(227..246)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     281..300
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    326..359
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(567..586)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgcggccgcg ttaacgctac catggagctc caaataatga ttttattttg actgatagtg
       61 acctgttcgt tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataat
      121 aaagttggaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      181 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      241 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      301 aatgtatctt atcatgtctg gatccgaagt tcctattctc tagaaagtat aggaacttct
      361 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
      421 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
      481 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
      541 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
      601 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
      661 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
      721 tg
//
