LOCUS       sequence-161345-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Calreticulin mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Del Cid N, Jeffery E, Rizvi SM, Stamper E, Peters LR, Brown WC, 
            Provoda C, Raghavan M
  TITLE     Modes of calreticulin recruitment to the major histocompatibility 
            complex class I assembly pathway.
  JOURNAL   J Biol Chem. 2010 Feb 12;285(7):4520-35. doi: 
            10.1074/jbc.M109.085407. Epub 2009 Dec 3.
  PUBMED    19959473
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.085407"; journalName: "J Biol Chem"; date: 
            "2010-02-12- 12"; volume: "285"; issue: "7"; pages: "4520-35"
COMMENT     Sequence Label: pmcsg7-mCRT(deltaP)
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg ggtaccgaga
       61 acctgtactt ccaatccaat gctatggccg cagaccctgc catctatttc aaagagcagt
      121 tcttggacgg agatgcctgg accaaccgct gggtcgaatc caaacataag tccgattttg
      181 gcaaatttgt cctcagttct ggcaaatttt acggggacct ggagaaggat aaagggctgc
      241 agacaagcca agatgcccga ttttacgcac tgtccgccaa attcgaaccc ttcagcaata
      301 agggccagac actggtggta cagttcacgg tgaagcatga gcagaatatc gactgtgggg
      361 gcggctacgt gaagctgttt ccgagtggct tggaccagaa ggacatgcat ggagactcag
      421 aatataacat catgtttggt ccggacatct gcggtcctgg caccaagaag gttcatgtca
      481 tctttaacta caagggcaag aatgtgctga tcaacaagga tatccggtgt aaggatgatg
      541 aattcacaca cctatacaca ctgattgtgc ggccagacaa cacctatgag gtgaaaattg
      601 acaacagcca ggtggagtca ggctccttgg aggatgattg ggactttctg ggaggacccg
      661 atgcaaatat ctatgcctat gatagttttg ctgtactggg cctagatctc tggcaggtca
      721 agtccgggac aatctttgac aatttcctca tcaccaatga tgaggcctat gcagaggagt
      781 ttggcaatga gacgtggggt gttaccaagg ctgcagagaa gcagatgaag gacaagcagg
      841 atgaggagca gaggcttaag gaagaagaag aggacaagaa gcgtaaagag gaagaagaag
      901 ctgaggataa agaggatgat gatgacagag atgaagatga ggatgaagaa gatgagaagg
      961 aggaagatga ggaaga
//
