LOCUS       sequence-161448-         548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for conjugational transfer into cyanobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Miao R, Liu X, Englund E, Lindberg P, Lindblad P
  TITLE     Isobutanol production in Synechocystis PCC 6803 using heterologous 
            and endogenous alcohol dehydrogenases.
  JOURNAL   Metab Eng Commun. 2017 Jul 29;5:45-53. doi: 
            10.1016/j.meteno.2017.07.003. eCollection 2017 Dec.
  PUBMED    29188183
REFERENCE   2  (bases 1 to 548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2017-07-29"; volume: "5:45-53. doi"; pages: " 
            10.1016/j.meteno.2017.07.003. eCollection 2017 Dec"
COMMENT     Sequence Label: pEEK2
FEATURES             Location/Qualifiers
     source          1..548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..43)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             53..61
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      183..226
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(230..248)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        316..420
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatccact tccgctaccc ttctcgaact gaggatgact ccacattaga aaacctcctt
       61 agcatgatta agatgtttca gtacgaaaat tgctttcatt gttgatctcc tttttaagtg
      121 aacttgggcc cttccacaca ttatattgtg agcgctcaca attgtcaaca gctcgaattc
      181 cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg cctcgtgata
      241 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      301 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      361 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      421 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      481 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      541 cgagtggg
//
