LOCUS       sequence-161626-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of CyOFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Chu J, Oh Y, Sens A, Ataie N, Dana H, Macklin JJ, Laviv T, Welf ES, 
            Dean KM, Zhang F, Kim BB, Tang CT, Hu M, Baird MA, Davidson MW, Kay 
            MA, Fiolka R, Yasuda R, Kim DS, Ng HL, Lin MZ
  TITLE     A bright cyan-excitable orange fluorescent protein facilitates 
            dual-emission microscopy and enhances bioluminescence imaging in 
            vivo.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3550.
  PUBMED    27240196
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3550."
COMMENT     Sequence Label: pNCS-CyOFP
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..722)
                     /codon_start=1
                     /product="orange-red fluorescent protein that is excitable 
                     by cyan light and suitable as an acceptor for blue-emitting
                     luciferase (Chu et al., 2016)"
                     /label=CyOFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     CDS             complement(join(729..743,744..752))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(756..788)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(792..809)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             829..851
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atcaagcttc gaattcatta cttgtacagc tcgtccatgc ctccgcccag gttggagtat
       61 ctggccacag cgtgctcgta ctgctcgacg taggtctcgt tgtcggcctc cttgattctt
      121 tccagtctgc ggtccacgta gtggacgccg ggcatcttca cgggtttctt ggacttgtat
      181 gtggtcttga agttgacgtg caggtggccc ccgcccacga gcttcagggc cttgtcgcat
      241 ctgccttcca ggccgccgtc agcggggtac atggtctcgg tgctgggctc ccagcccagt
      301 gttttcttct gcatcacggg gccgttggct gggaagttca cccctctgac cttgacgttg
      361 tagatgagct cgccgtcctg gaggctggtg tcctgggtgg cggtcagcac gcccccgtct
      421 tcgaacacca tgactctctc ccatgtgaag ccctcaggga aggactgctt aaaataatcg
      481 gggaggtcgg cggggtactt gatgaacacc ttgctcccgt acataaagtg ggtggccagg
      541 atgtcgaatg cgaacggcag ggggcctccc tccaccacct tgatcctgtt ggtctgcttg
      601 ccctcgtagg gcttgccctc cccttcgtgg gtgcacttga actggtggcc gttcacgctg
      661 ccttccaggt acagcttgct tctcatgttc tccttgatca gctcctcgcc cttgctcacc
      721 atcggatcct tatcgtcatc gtcgtacaga tcccgaccca tttgctgtcc accagtcatg
      781 ctagccatac catgatgatg atgatgatga gaaccccgca tatgtatatc tccttcttaa
      841 agttaaacaa aacagtactt tcctattata tcattcatcg catcaggtgt caagaataaa
      901 ctcggtgatt ctag
//
