LOCUS       sequence-161637-        4668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KEAP1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_KEAP1_p.P278S
FEATURES             Location/Qualifiers
     source          1..4668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1023..1122)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1139..1158)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1140..1158)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1163..1179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1163..1179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1610..2401
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2494..3082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2983..3002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3236..3253
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3412..3439)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3531..3617)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3666..3688
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3680..3697
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3681..3697
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3713..3812
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcagaagtgc gagatcctgc agtccgactc ccgctgcaag gactacctgg tcaagatctt
       61 cgaggagctc accctgcaca agcccacgca ggtgatgccc tgccgggcgc ccaaggtggg
      121 ccgcctgatc tacaccgcgg gcggctactt ccgacagtcg ctcagctacc tggaggctta
      181 caaccccagt gacggcacct ggctccggtt ggcggacctg caggtgccgc ggagcggcct
      241 ggccggctgc gtggtgggcg ggctgttgta cgccgtgggc ggcaggaaca actcgcccga
      301 cggcaacacc gactccagcg ccctggactg ttacaacccc atgaccaatc agtggtcgcc
      361 ctgcgccccc atgagcgtgc cccgtaaccg catcggggtg ggggtcatcg atggccacat
      421 ctatgccgtc ggcggctccc acggctgcat ccaccacaac agtgtggaga ggtatgagcc
      481 agagcgggat gagtggcact tggtggcccc aatgctgaca cgaaggatcg gggtgggcgt
      541 ggctgtcctc aatcgtctcc tttatgccgt ggggggcttt gacgggacaa accgccttaa
      601 ttcagctgag tgttactacc cagagaggaa cgagtggcga atgatcacag caatgaacac
      661 catccgaagc ggggcaggcg tctgcgtcct gcacaactgt atctatgctg ctgggggcta
      721 tgatggtcag gaccagctga acagcgtgga gcgctacgat gtggaaacag agacgtggac
      781 tttcgtagcc cccatgaagc accggcgaag tgccctgggg atcactgtcc accaggggag
      841 aatctacgtc cttggaggct atgatggtca cacgttcctg gacagtgtgg agtgttacga
      901 cccagataca gacacctgga gcgaggtgac ccgaatgaca tcgggccgga gtggggtggg
      961 cgtggctgtc accatggagc cctgccggaa gcagattgac cagcagaact gtaccagttg
     1021 attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1081 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1141 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1201 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     1261 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     1321 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     1381 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1441 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     1501 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1561 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     1621 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1681 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1741 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1801 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1861 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1921 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1981 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2041 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2101 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2161 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2221 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2281 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2341 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2401 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2461 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2521 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2581 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2641 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2701 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2761 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2821 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2881 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2941 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3001 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3061 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3121 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3181 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3241 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3301 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3361 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
     3421 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
     3481 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     3541 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
     3601 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
     3661 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat
     3721 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
     3781 tataatgcca actttgtaca aaaaagttgg caccatgcag ccagatccca ggcctagcgg
     3841 ggctggggcc tgctgccgat tcctgcccct gcagtcacag tgccctgagg gggcagggga
     3901 cgcggtgatg tacgcctcca ctgagtgcaa ggcggaggtg acgccctccc agcatggcaa
     3961 ccgcaccttc agctacaccc tggaggatca taccaagcag gcctttggca tcatgaacga
     4021 gctgcggctc agccagcagc tgtgtgacgt cacactgcag gtcaagtacc aggatgcacc
     4081 ggccgcccag ttcatggccc acaaggtggt gctggcctca tccagccctg tcttcaaggc
     4141 catgttcacc aacgggctgc gggagcaggg catggaggtg gtgtccattg agggtatcca
     4201 ccccaaggtc atggagcgcc tcattgaatt cgcctacacg gcctccatct ccatgggcga
     4261 gaagtgtgtc ctccacgtca tgaacggtgc tgtcatgtac cagatcgaca gcgttgtccg
     4321 tgcctgcagt gacttcctgg tgcagcagct ggaccccagc aatgccatcg gcatcgccaa
     4381 cttcgctgag cagattggct gtgtggagtt gcaccagcgt gcccgggagt acatctacat
     4441 gcattttggg gaggtggcca agcaagagga gttcttcaac ctgtcccact gccaactggt
     4501 gaccctcatc agccgggacg acctgaacgt gcgctgcgag tccgaggtct tccacgcctg
     4561 catcaactgg gtcaagtacg actgcgaaca gcgacggttc tacgtccagg cgctgctgcg
     4621 ggccgtgcgc tgccactcgt tgacgtcgaa cttcctgcag atgcagct
//
