LOCUS       sequence-161703-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of RPL3promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP80
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(227..937)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 gcatgcattt aaatcattac gaccgagatt cccgggtaat aactgatata attaaattga
       61 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
      121 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
      181 cttagcatcc cttccctttg caaatagtcc tactagttta agatcttttg tacaattcat
      241 ccataccatg ggtaatacca gcagcagtaa caaattctaa caagaccatg tggtctctct
      301 tttcgtttgg atctttggat aaggcagatt gagtggataa gtaatggttg tctggtaaca
      361 agactggacc atcaccaatt ggagtatttt gttgataatg gtcagctaat tgaacagaac
      421 catcttcaat gttgtgtcta attttgaagt taactttgat accattcttt tgtttgtcag
      481 ccatgatgta aacattgtga gagttatagt tgtattccaa tttgtgacct aaaatgttac
      541 catcttcttt aaaatcaata ccttttaatt cgattctatt aactaaggta tcaccttcaa
      601 acttgacttc agctctggtc ttgtagttac cgtcatcttt gaaaaaaata gttctttctt
      661 gaacataacc ttctggcatg gcagacttga aaaagtcatg ttgtttcata tgatctgggt
      721 atctcgcaaa acattgaaca ccataaccga aagtagtgac taaggttggc catggaactg
      781 gcaatttacc agtagtacaa ataaatttta aggtcaattt accgtaagta gcatcacctt
      841 caccttcacc ggagacagaa aatttgtgac cattaacatc accatctaat tcaaccaaaa
      901 ttgggacaac accagtgaat aattcttcac ctttagagga tcccatgatt gattgttgta
      961 gtaactgtgt tgttctagag atgacaaaac cgaaatgaaa taatgagtaa agaaagatga
     1021 ggattgacaa agacaactaa ataaatcgt
//
