LOCUS       sequence-161710-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of YEF3 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFP82
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    677..710
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacatcacc atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt
       61 tagaggatcc catcttttaa tgttatcgat ggattcgaac tagaaaaata cacggttctt
      121 tagaaaacaa ttaaggaaag aaagagagcg taagaaaaag agatattcta caaaattttg
      181 atgcaaaagg tttttataaa acttcttatc ttatatgaaa gatgagatga gataattata
      241 ctcgaggaag cgaaaaaatt ttcaccaacg caaaaaaaga aaaagaaaaa aaaaaaaaaa
      301 acattaaaaa cgtttctttt tcacgtgcta agtgatctaa taatttaggg ttatgcatgg
      361 gtggggccgg ataaaccgcg gttgaaagtg attaaacgga gtcttttctt ttttttgctt
      421 cttcgtttct ttttttaatt tcttctttct tcacgaatat ttattaccca gcctgtcatg
      481 attttttgat acgtaattgc cctggttggg cccttttgcg agtggcagta agtatggaag
      541 gggcgaacaa tgattgtaag ttgttttttt aatagggtaa cgaccgggta aaatagaaag
      601 aagtgttgac taagaaacgg gaatcaacat tcacttgctt atcttaaaat gttatgtatg
      661 tcgagcaacc cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga
      721 gatcccaata caacagatca cgtgatcttt tgtaagatga agttgaagta gtgttgcacc
      781 gtgccaatgc aggtggctat tagattaaat atgtgatttg ttctattaag tttcctgtat
      841 aattaatggg gagcgctgat tctcttttgg tacgcttccc atccagcatt tctgtatctt
      901 tcaccttcaa ccttaggatc tctacccttg gcgaaaagtc ctctgccaac aatgatga
//
