LOCUS       sequence-161729-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to evaluate the expression output of TEF1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFPE9
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(97..510)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     protein_bind    721..754
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct
       61 ttagaggatc ccattttctc gagtttgtaa ttaaaactta gattagattg ctatgctttc
      121 tttctaatga gcaagaagta aaaaaagttg taatagaaca agaaaaatga aactgaaact
      181 tgagaaattg aagaccgttt attaacttaa atatcaatgg gaggtcatcg aaagagaaaa
      241 aaatcaaaaa aaaaaatttt caagaaaaag aaacgtgata aaaattttta ttgccttttt
      301 cgacgaagaa aaagaaacga ggcggtctct tttttctttt ccaaaccttt agtacgggta
      361 attaacgaca ccctagagga agaaagaggg gaaatttagt atgctgtgct tgggtgtttt
      421 gaagtggtac ggcgatgcgc ggagtccgag aaaatctgga agagtaaaaa aggagtagaa
      481 acattttgaa gctatggtgt gtgggggatc acttgtgggg gattgggtgt gatgtaagga
      541 ttcgcggtcc tcgaaaatta aaagtccaac gcgcctgttg cttcctatgt gatatgtatt
      601 atatgtaata tgcataaata tatctactgc attgtatttt gaacgtacaa agtatgcatt
      661 gtttatacgc tattatcagc caaagttggg tggtcgcttt ctggtcgagc aacccttaat
      721 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tagagatccc aatacaacag
      781 atcacgtgat cttttgtaag atgaagttga agtagtgttg caccgtgcca atgcaggtgg
      841 ctattagatt aaatatgtga tttgttctat taagtttcct gtataattaa tggggagcgc
      901 tgattctctt ttggtacgct tcccatccag catttctgta tctttcacct tcaaccttag
      961 gatctctacc cttggcgaaa agtcctctgc caacaatgat gatatctgat ccaccactta
//
