LOCUS       sequence-161777-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NowGFP gene for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Sarkisyan KS, Goryashchenko AS, Lidsky PV, Gorbachev DA, Bozhanova 
            NG, Gorokhovatsky AY, Pereverzeva AR, Ryumina AP, Zherdeva VV, 
            Savitsky AP, Solntsev KM, Bommarius AS, Sharonov GV, Lindquist JR, 
            Drobizhev M, Hughes TE, Rebane A, Lukyanov KA, Mishin AS
  TITLE     Green fluorescent protein with anionic tryptophan-based chromophore 
            and long fluorescence lifetime.
  JOURNAL   Biophys J. 2015 Jul 21;109(2):380-9. doi: 10.1016/j.bpj.2015.06.018.
  PUBMED    26200874
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2015.06"; journalName: "Biophys J"; date: 
            "2015-07-21- 21"; volume: "109"; issue: "2"; pages: "380-9"
COMMENT     Sequence Label: NowGFP /pQE-30
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(57..151)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(173..886,887..889))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEKLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKMSLK
                     FICTTGKLPVPWPTLKTTLTWGMQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNAISGNANITADKQKNGIKA
                     YFTIRHDVEDGSVLLADHYQQNTPIGDGPVLLPDNHYLSTQSKQSKDPNEKRDHMVLLE
                     FVTAAGIPLGADELYK"
     primer_bind     complement(218..239)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     567..586
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     826..847
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tttttttctc cattttagct tccttagctc ctgaaaatct cgccaagcta gcttggattc
       61 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
      121 gaggtcatta ctggatctat caacaggagt ccaagctcag ctaattaagc ttttacttgt
      181 acagctcgtc cgcgccgaga gggatcccgg cggcggtcac gaactccagc aggaccatgt
      241 gatcgcgctt ctcgttgggg tctttgctct gcttggactg ggtgctcagg tagtggttgt
      301 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagca
      361 gcacgctgcc gtcctcgacg tcgtggcgga tcgtgaagta ggccttgatg ccgttcttct
      421 gcttgtcggc ggtgatattg gcgttgccgc tgatggcgtt gtactccagc ttgtgcccca
      481 ggatgttgcc gtcctccttg aagtcgacgc ccttcagctc gatgcggttc accagggtgt
      541 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
      601 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      661 ggtcggggta gcgggcgaag cactgcatgc cccaggtcag ggtggttttg agggtgggcc
      721 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggacatcttg ccgtaggtgg
      781 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
      841 cgaccaggat gggcaccacc ccggtgaaca gcttctcgcc cttgctcacg gatccgtgat
      901 ggtgctggtg atgcgatcct ctcat
//
