LOCUS       sequence-161803-        4581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ABCB9, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ABCB9_p.R281L
FEATURES             Location/Qualifiers
     source          1..4581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             374..388
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     protein_bind    complement(1981..2080)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2097..2116)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2098..2116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2121..2137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2121..2137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2568..3359
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3452..4040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3941..3960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4194..4211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4370..4397)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4489..4575)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcggctgtgg aaggcggtgg tggtgacttt ggccttcatg agtgtggaca
      241 tctgcgtgac cacggccatc tatgtcttca gccacctgga ccgcagcctc ctggaggaca
      301 tccgccactt caacatcttt gactcggtgc tggatctctg ggcagcctgc ctgtaccgca
      361 gctgcctgct gctgggagcc accattggtg tggccaagaa cagtgcgctg gggccccggc
      421 ggctgcgggc ctcgtggctg gtcatcaccc tcgtgtgcct cttcgtgggc atctatgcca
      481 tggtgaagct gctgctcttc tcagaggtgc gcaggcccat ccgggacccc tggttttggg
      541 ccctgttcgt gtggacgtac atttcactcg gcgcatcctt cctgctctgg tggctgctgt
      601 ccaccgtgcg gccaggcacc caggccctgg agccaggggc ggccaccgag gctgagggct
      661 tccctgggag cggccggcca ccgcccgagc aggcgtctgg ggccacgctg cagaagctgc
      721 tctcctacac caagcccgac gtggccttcc tcgtggccgc ctccttcttc ctcatcgtgg
      781 cagctctggg agagaccttc ctgccctact acacgggccg cgccattgat ggcatcgtca
      841 tccagaaaag catggatcag ttcagcacgg ctgtcgtcat cgtgtgcctg ctggccattg
      901 gcagctcatt tgccgcaggt attcggggcg gcatttttac cctcatattt gccagactga
      961 acattcgcct tcgaaactgt ctcttccgct cactggtgtc ccaggagaca agcttctttg
     1021 atgagaacct cacaggggac ctcatctccc gcctgacctc ggacaccacc atggtcagcg
     1081 acctggtctc ccagaacatc aatgtcttcc tgcggaacac agtcaaggtc acgggcgtgg
     1141 tggtcttcat gttcagcctc tcatggcagc tctccttggt caccttcatg ggcttcccca
     1201 tcatcatgat ggtgtccaac atctacggca agtactacaa gaggctctcc aaagaggtcc
     1261 agaatgccct ggccagagcg agcaacacgg cggaggagac catcagtgcc atgaagactg
     1321 tccggagctt cgccaatgag gaggaggagg cagaggtgta cctgcggaag ctgcagcagg
     1381 tgtacaagct gaacaggaag gaggcagctg cctacatgta ctacgtctgg ggcagcgggc
     1441 tcacactgct ggtggtccag gtcagcatcc tctactacgg gggccacctt gtcatctcag
     1501 gccagatgac cagcggcaac ctcatcgcct tcatcatcta cgagtttgtc ctgggagatt
     1561 gtatggagtc cgtgggctcc gtctacagtg gcctgatgca gggagtgggg gctgctgaga
     1621 aggtgttcga gttcatcgac cggcagccga ccatggtgca cgatggcagc ttggcccccg
     1681 accacctgga gggccgggtg gactttgaga atgtgacctt cacctaccgc actcggcccc
     1741 acacccaggt cctgcagaat gtctccttca gcctgtcccc cggcaaggtg acggccctgg
     1801 tggggccctc gggcagtggg aagagctcct gtgtcaacat cctggagaac ttctaccccc
     1861 tggagggggg ccgggtgctg ctggacggca agcccatcag cgcctacgac cacaagtact
     1921 tgcaccgtgt ggtatgtgca cgggcctggg ccacacttct ccgccctttc tgcatctgat
     1981 tgccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     2041 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     2101 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     2161 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     2221 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     2281 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2341 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2401 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2461 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2521 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2581 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2641 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2701 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2761 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2821 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2881 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2941 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     3001 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     3061 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     3121 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     3181 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3241 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3301 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3361 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3421 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3481 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3541 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3601 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3661 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3841 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3901 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3961 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4021 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4081 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4141 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4201 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4261 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4321 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     4381 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     4441 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     4501 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     4561 ctttcgtttt atttgatgcc t
//
