LOCUS       sequence-161836-       12378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-SLITRK3 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 12378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-SLITRK3
FEATURES             Location/Qualifiers
     source          1..12378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..3083
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4126..4167
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7070..7658
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7123..7143)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7541..7552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7661..7677)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7662..7678)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8183..8363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8389..8406)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8395..8619
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8665..9093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8752..8771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8962..8983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9102..9122)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9107..9436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9287..9422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9349..9368
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9484..9531
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9550..9924
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10054..10175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10091..10110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10145..10164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10224..10240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10224..10240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10237..10259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10248..10264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10272..10278,10279..10296,10297..10302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10317..10338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10455..10472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10626..11214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10706..10725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11385..12176,12177..12245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12008..12027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12246..12350)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagcacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta ataccgccat gcatctgcag agggccctgc gtatgagtgc
     2641 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
     2701 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
     2761 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     2821 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     2881 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     2941 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     3001 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     3061 tcgtgtcgtg cctgagagcg cagtctagag gatcccgcca ccatgaaacc ttccatagct
     3121 gagatgcttc acagaggaag gatgttgtgg ataattcttc taagcacaat tgctctagga
     3181 gctagccagc ttactccaac tttctacgat aactcttgtc ctaacgtgag caacatcgtg
     3241 cgagatacta tcgtgaacga gttgcggtct gatccgagga tagccgcttc aattttgcgg
     3301 ctgcatttcc acgattgttt tgtaaacggc tgtgatgctt ccatcctcct cgacaatacc
     3361 acctctttca ggactgaaaa ggatgccttc ggtaacgcga atagtgctag agggtttccc
     3421 gtgatcgata gaatgaaagc cgctgtggag agcgcctgcc cccggacagt tagttgtgca
     3481 gaccttctga ccatagcggc ccagcaatcc gtgacgctgg ccggtggccc cagctggagg
     3541 gtccccctgg gaaggaggga ctctctgcag gcttttctgg acttggcaaa cgcaaacctg
     3601 cctgctccct tcttcaccct tccccagctg aaggattcct tcaggaatgt cggcctgaac
     3661 cgaagtagtg atttggtggc tctgtccgga ggccacacat tcggcaagaa tcagtgcagg
     3721 ttcatcatgg acagactgta taacttttcc aacaccggct tgcctgaccc taccctgaac
     3781 acaacatact tgcagactct cagagggctg tgtccgctca atggcaactt gtctgcactg
     3841 gttgactttg acctgcgcac cccaaccatc ttcgacaata agtattacgt caatctggag
     3901 gaacagaagg gcctgattca gtccgaccag gagctgttca gttccccaaa tgctaccgac
     3961 actattcctc ttgtgcgcag cttcgctaat agcacacaaa cctttttcaa tgctttcgtg
     4021 gaagctatgg atcggatggg aaatataacg cccctgacgg gtacgcaggg acagataaga
     4081 ctgaactgta gggtcgtgaa cagcaactct ggctcgggct cgggcggcaa gcccatcccc
     4141 aaccccctgc tgggcctgga cagcaccacc ggttggacta ccccgattcc cctaatagag
     4201 gactcagagg aaatagatga gccctgtttt gatccatgct actgtgaagt taaagaaagc
     4261 ctctttcata tacattgtga cagtaaagga tttacaaata ttagtcagat taccgagttc
     4321 tggtcaagac cttttaaact gtatctgcag aggaattcta tgaggaaatt atataccaac
     4381 agttttcttc atttgaataa tgctgtgtct attaatcttg ggaacaatgc attgcaggac
     4441 attcagactg gagctttcaa tggtcttaag attttaaaga gactatatct acatgaaaac
     4501 aaactagatg tcttcagaaa tgacaccttc cttggcttgg aaagtctaga atatctgcag
     4561 gcagattaca atgtcattaa acgtattgag agtggggcat ttcggaacct aagtaaattg
     4621 agggttctga ttttaaatga taatctcatc cccatgcttc caaccaattt atttaaggct
     4681 gtctctttaa cccatttgga cctacgtgga aataggttaa aggttctttt ttaccgagga
     4741 atgctagatc acattggcag aagcctgatg gagctccagc tggaagaaaa cccttggaac
     4801 tgtacatgtg aaattgtaca actgaagagt tggctggaac gcattcctta tactgccctg
     4861 gtgggagaca ttacctgtga gacccctttc cacttccatg gaaaggacct acgagaaatc
     4921 aggaagacag aactctgtcc cttgttgtct gactctgagg tagaggctag tttgggaatt
     4981 ccacattcgt catcaagtaa ggagaatgca tggccaacta agccttcctc aatgctatcc
     5041 tctgttcatt ttactgcttc ttctgtcgaa tacaagtcct caaataaaca gcctaagccc
     5101 accaaacagc ctcgaacacc aaggccaccc tccacctccc aagctttata tcctggtcca
     5161 aaccagcctc ccattgctcc ttatcagacc agaccaccaa tccccattat atgccccact
     5221 gggtgtacct gtaatttgca catcaatgac cttggcttga ctgtcaactg caaagagcga
     5281 ggatttaata acatttctga acttcttcca aggcccttga atgccaagaa actgtatctg
     5341 agtagcaatc tgattcagaa aatataccgt tctgattttt ggaatttttc ttccttggat
     5401 ctcttgcatc tggggaacaa tcgtatttcc tatgtccaag atggggcctt tatcaacttg
     5461 cccaacttaa agagcctctt ccttaatggc aacgatatag agaagctgac accaggcatg
     5521 ttccgaggcc tacagagttt gcactacttg tactttgagt tcaatgtcat ccgggaaatc
     5581 cagcctgcag ccttcagcct catgcccaac ttgaagctgc tattcctcaa taataactta
     5641 ctgaggactc tgccaacaga cgcctttgct ggcacatccc tggcccggct caacctgagg
     5701 aagaactact tcctctatct tcccgtggct ggtgtcctgg aacacttgaa tgccattgtc
     5761 cagatagacc tcaatgagaa tccttgggac tgcacctgtg acctggtccc ctttaaacag
     5821 tggatcgaaa ccatcagctc agtcagtgtg gttggtgatg tgctttgcag gagccctgag
     5881 aacctcacgc accgtgatgt gcgcactatt gagctggaag ttctttgccc agagatgctg
     5941 cacgttgcac cagctggaga atccccagcc cagcctggag attctcacct tattggggca
     6001 ccaaccagtg catcacctta tgagttttct cctcctgggg gccctgtgcc actttctgtg
     6061 ttaattctca gcctgctggt tctgtttttc tcagcagtct ttgttgctgc aggcctcttt
     6121 gcctacgtgc tccgaaggcg tcgaaagaag ctgcccttca gaagcaagcg gcaggaaggt
     6181 gtggacctta ctggcatcca aatgcaatgc cacaggctgt ttgaggatgg tggaggtggt
     6241 ggtggcggaa gtgggggtgg tggtcgacca actctttcct ctccagagaa ggcccctccc
     6301 gtgggtcatg tgtatgagta catcccccac ccggttaccc aaatgtgcaa caaccccatc
     6361 tacaagcctc gtgaggagga ggaggtggct gtttcatcag cccaagaagc agggagtgca
     6421 gaacgtgggg gtccagggac acaaccaccg ggaatgggtg aggctctcct aggaagtgag
     6481 cagtttgctg agacacccaa ggagaaccat agtaactacc ggaccttgct ggaaaaagag
     6541 aaggagtggg ccctagcagt gtccagctcc cagcttaaca ccatagtgac ggtgaatcac
     6601 catcaccctc accacccagc agttggtggg gtttcaggag tagttggggg aactggggga
     6661 gacttggcag ggttccgcca ccatgagaaa aatggtgggg tggtgctgtt tcctcctggg
     6721 ggaggctgtg gtagtggcag tatgctacta gatcgagaga ggccacagcc tgccccctgc
     6781 acagtgggat ttgtggactg tctctatgga acagtgccca aattaaagga actgcacgtg
     6841 caccctcctg gcatgcaata cccagactta cagcaggatg ccaggctcaa agaaaccctt
     6901 ctcttctcgg ctggaaaggg cttcacagac caccaaaccc aaaaaagtga ttacctcgag
     6961 ttaagggcca aacttcaaac caagccggat tacctcgaag tcctggagaa gacaacatac
     7021 aggttctaag gcgcgcccct gcagggaatt cgatatcaag cttatcgata atcaacctct
     7081 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7141 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7201 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7261 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7321 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7381 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7441 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7501 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7561 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7621 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
     7681 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     7741 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     7801 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7861 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     7921 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     7981 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     8041 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     8101 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     8161 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8221 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8281 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8341 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     8401 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     8461 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     8521 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     8581 acaatagcag gcatgctggg gatgcggcgg gctctatggc ttctgaggcg gaaagaacca
     8641 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8701 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8761 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8821 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8881 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8941 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9001 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9061 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     9121 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9181 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9241 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     9301 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9361 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9421 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     9481 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     9541 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     9601 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     9661 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     9721 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     9781 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     9841 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     9901 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     9961 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    10021 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    10081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10141 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    10201 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10261 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10321 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10381 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10441 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10501 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10561 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10621 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10681 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10741 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10801 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10861 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10921 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10981 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11041 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    11101 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11161 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    11221 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11281 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    11341 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11401 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11461 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11521 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11581 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11641 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11701 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11761 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11821 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11881 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11941 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    12001 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    12061 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    12181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    12241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    12301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    12361 cgaaaagtgc cacctgac
//
