LOCUS       sequence-161859-        4311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ATF2, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ATF2_p.A481V
FEATURES             Location/Qualifiers
     source          1..4311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2190..2289)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2306..2325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2307..2325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2330..2346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2330..2346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2777..3568
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3661..4249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4150..4169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaaattca agttacatgt gaattctgcc aggcaataca aggacctgtg
      721 gaatatgagt gatgacaaac cctttctatg tactgcgcct ggatgtggcc agcgttttac
      781 caacgaggat catttggctg tccataaaca taaacatgag atgacactga aatttggtcc
      841 agcacgtaat gacagtgtca ttgtggctga tcagacccca acaccaacaa gattcttgaa
      901 aaactgtgaa gaagtgggtt tgtttaatga gttggcgagt ccatttgaga atgaattcaa
      961 gaaagcttca gaagatgaca ttaaaaaaat gcctctagat ttatcccctc ttgcaacacc
     1021 tatcataaga agcaaaattg aggagccttc tgttgtagaa acaactcacc aggatagtcc
     1081 tttacctcac ccagagtcta ctaccagtga tgagaaggaa gtaccattgg cacaaactgc
     1141 acagcccaca tcagctattg ttcgtccagc atcattacag gttcccaatg tgctgcttac
     1201 aagttctgac tcaagtgtaa ttattcagca ggcagtacct tcaccaacct caagtactgt
     1261 aatcacccag gcaccatcct ctaacaggcc aattgtccct gtaccaggcc catttcctct
     1321 tctgttacat cttcctaatg gacaaaccat gcctgttgct attcctgcat caattacaag
     1381 ttctaatgtg catgttccag ctgcagtccc actcgttcga ccagtcacca tggtgcctag
     1441 tgttccagga atcccaggtc cttcctctcc ccaaccagta cagtcagaag caaaaatgag
     1501 attaaaagct gctttgaccc agcaacatcc tccagttacc aatggtgata ctgtcaaagg
     1561 tcatggtagc ggattggtta ggactcagtc agaggaatct cgaccgcagt cattacaaca
     1621 gccagccaca tccactacag aaactccggc ttctccagct cacacaactc cacagaccca
     1681 aagtacaagt ggtcgtcgga gaagagcagc taacgaagat cctgatgaaa aaaggagaaa
     1741 gtttttagag cgaaatagag cagcagcttc aagatgccga caaaaaagga aagtctgggt
     1801 tcagtcttta gagaagaaag ctgaagactt gagttcatta aatggtcagc tgcagagtga
     1861 agtcaccctg ctgagaaatg aagtggcaca gctgaaacag cttcttctgg ctcataaaga
     1921 ttgccctgta accgccatgc agaagaaatc tggctatcat actgctgata aagatgatag
     1981 ttcagaagac atttcagtgc cgagtagtcc acatacagaa gctatacagc atagttcggt
     2041 cagcacatcc aatggagtca gttcaacctc caaggcagaa gctgtagcca cttcagtcct
     2101 cacccagatg gtggaccaga gtacagagcc tgctctttca cagatcgtta tggctccttc
     2161 ctcccagtca cagccctcag gaagttgatt gccaactttc ttgtacaaag ttgtcattat
     2221 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2281 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2341 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2401 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     2461 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     2521 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     2581 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2641 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     2701 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     2761 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     2821 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     2881 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     2941 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     3001 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     3061 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     3121 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     3181 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     3241 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     3301 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     3361 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     3421 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     3481 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     3541 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     3601 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3781 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
