LOCUS       sequence-161937-        4887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ZBTB24, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ZBTB24_p.K123M
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2766..2865)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2882..2901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2883..2901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2906..2922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2906..2922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3353..4144
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4237..4825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4726..4745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcagaaa catcgccaga gccttctggg cagcttgttg tacactcaga
      721 cgctcacagt gacactgtgc tggccagttt tgaggatcag aggaagaaag gcttcctctg
      781 tgacattact ttaatcgtgg agaatgtaca tttccgggcc cacaaagcct tacttgctgc
      841 cagtagtgaa tacttctcaa tgatgtttgc agaagagggg gaaatcggcc aatccattta
      901 tatgctggaa ggcatggttg cagacacctt tggtatcctg ctggaattta tctacacagg
      961 ttatctccat gccagtgaga aaagtacaga acaaatcctg gctactgctc agttcttaaa
     1021 agtctatgac ctggtaatgg cttacacaga cttccaaaat aatcatagct ccccaaagcc
     1081 aacaactttg aacactgctg gtgccccagt ggttgttatc tctaataaga aaaacgatcc
     1141 tccaaagcgg aaacggggaa gaccaaaaaa agtcaataca ttgcaggagg agaaatcaga
     1201 actggctgca gaggaagaaa tacagttaag agtgaacaat tcagttcaga atagacaaaa
     1261 ctttgtggtt aaaggagaca gtggtgtact gaatgagcaa attgcagcaa aagaaaagga
     1321 agaatcggag cctacttgtg agccaagtag agaggaggaa atgccagttg aaaaagatga
     1381 gaactatgat cccaagaccg aggatggcca ggcaagccag agtcgataca gcaagcggag
     1441 gatttggaga tccgtcaaac ttaaagatta caaacttgtt ggggatcaag aggaccatgg
     1501 ttcagccaag aggatctgtg gaaggagaaa gcgccctgga ggccctgagg cccgctgtaa
     1561 agactgtggc aaggtcttta agtacaatca ctttttagca atccaccaga ggagccacac
     1621 aggggagcga cctttcaaat gtaatgagtg tggaaaaggc tttgcccaga agcactcgct
     1681 acaggtccac accaggatgc acacaggcga gcggccgtac acctgcaccg tgtgcagcaa
     1741 ggctctgacc accaagcact cactgctgga gcacatgagc ctgcactcag gacagaagtc
     1801 ttttacctgt gatcaatgcg gaaaatattt cagccagaac agacagctaa agagccatta
     1861 ccgagttcat acaggccact cattaccgga atgcaaagac tgccatcgca aattcatgga
     1921 tgtgtctcag ctaaagaaac atctgcgaac acacacaggt gagaagccat ttacttgtga
     1981 aatctgtggc aaatctttca cagcaaagag ttctcttcag acccacatca gaatccatcg
     2041 aggagaaaag ccatactcct gtggcatttg tggcaaatcc ttctctgact ccagtgccaa
     2101 aaggagacac tgcattctac acactggcaa gaagcctttc tcctgccctg agtgtaactt
     2161 acagtttgct cgcttagaca acttgaaggc tcacttgaaa attcatagca aggagaagca
     2221 tgcttcagat gccagcagca tttctggcag tagtaataca gaagaggtca ggaatattct
     2281 tcagctacag ccatatcaac tctctacctc gggagagcag gaaattcagc ttctcgtaac
     2341 cgattctgta cataacatca atttcatgcc cggtcctagc cagggaatca gcattgtgac
     2401 tgcagagagt tcccaaaaca tgactgcaga ccaggctgct aatcttaccc tgctcacgca
     2461 gcagccagag caactgcaga atttaattct ttcagctcaa caggagcaaa cagaacacat
     2521 tcagagcctc aatatgattg aaagccagat ggggccctca caaacagagc cagtgcacgt
     2581 gatcactctg tccaaggaaa cactggaaca tcttcatgcc catcaagagc aaacagagga
     2641 gctccattta gctacaagta cttcagatcc agctcagcac ctgcagctga cacaggagcc
     2701 tggtccgcca ccacccactc accacgtgcc ccagccaacg ccacttggcc aggagcagag
     2761 ctgattgcca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     2821 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     2881 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2941 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     3001 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     3061 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     3121 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     3181 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     3241 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     3301 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     3361 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     3421 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     3481 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     3541 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     3601 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     3661 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     3721 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     3781 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3841 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     3901 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     3961 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     4021 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     4081 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     4141 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     4201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4381 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4861 cttttgctgg ccttttgctc acatgtt
//
