LOCUS       sequence-161940-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-Late1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-01
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(153..182)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(904..923)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(905..923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(968..1007)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tgagaagacg ggtcgttacc gaacagagaa cgcagagagg tcagcgggta cagttctttg
       61 tcgatcggct cctgtttttg cggtggcgtg gtggtttcaa caccagaacg gaaagattct
      121 tccggcggtg cggtcggttc cggacgagac tgcagatctt cttcgctaat cagtttctgt
      181 tcggtgcagc cacgaatctt ctcaacgaac gccttggcct tttccgccac ttccaccggg
      241 gtgcctttca ctaacgcgct gcccacgccc acggccagca cgcccgcttt gaaccattcg
      301 cacacattgt ccagattaac accgccggtt ggcacaaatt tcacattcgg aaacggacct
      361 ttcatcgctt taacaaactg cggacccacc acctcacccg gaaacagttt taagatggta
      421 tggcccagtt tcatggcttt caccagttcg gtcggggtca taacgcctgg catatagaac
      481 acacccttct ccttgcaaaa ttggctaatt tcctcgtcca ggtgcggact cacaatgaat
      541 tcggcgccac tttccaccgc tttacggcac tgctccacgc tggtcacggt gcccgcacca
      601 ataatcgcgc ccatttcctt cagaaagctc agttccttaa tcacggtatc cgcatccggc
      661 acggtaaagg taatttcaat cagatgaacg ccgcccagga aaaccgccag cgctttcttt
      721 ttcgcttctt ccacactatt ggcacgcagc acggcaacga tcttgtgttt cttgaacagc
      781 tcttcgatct tgctaccaga gtcagagccg gagccagatt tagaaccgga cgcacgcgca
      841 cccatggtac ctccggatcg ataattctag agggcccgtt taaacgctag ccagcttggg
      901 tctccctata gtgagtcgta ttaatttcga taagcnagta agcagtgggt tctctagtta
      961 gccaganagc tctgcttata tagancctcc caccgtacac gcctaccgnc cantttgcgt
     1021 cantggggcg gaa
//
