LOCUS       sequence-162044-        4862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pACSA_PEZ4_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ11
FEATURES             Location/Qualifiers
     source          1..4862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(729..747)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      743..790
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1294..1313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1295..1313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1320..1337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1320..1336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1329..1351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1477..1932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1564..1583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1774..1795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2010..2114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2115..2183,2184..2975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPEMNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2333..2352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3146..3734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3635..3654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3888..3905
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4022..4043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4058..4063,4064..4081,4082..4088)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4096..4112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4101..4123
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4120..4136
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4120..4136
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4154..4172
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4154..4171
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atgtcggtca tcaagcccga aatgaaaatt aaactgcgca tggagggcgc tgtgaacggg
       61 cacaagttcg tgatcgaagg tgaaggcatt ggtaagccgt atgaaggtac tcaaaccctg
      121 gacttaacgg ttaaagaagg tgccccgttg ccatttagct acgacatcct gacacccgct
      181 tttcagtatg gcaatcgcgc gttcaccaag tatccgaaag atattccgga ttatttcaag
      241 caggcattcc cggaaggata ctcatgggaa cgctcgatga cctacgaaga ccagggcatt
      301 tgcattgcca caagcgacat cacaatggag ggtgattgct ttttttacaa aattcgcttc
      361 gacgggacaa acttcccgcc gaatggccca gtgatgcaga aaaagacgct taaatgggaa
      421 ccgtcgacag agaaaatgta cgttcgcgat ggcgttctga aaggcgatgt taatatggcg
      481 ctgcttctcg aagggggagg ccattaccgt tgtgatttca aaaccacata caaagcaaaa
      541 aaagatgttc gtttgccgga cgcgcatgaa gtcgatcacc gcattgagat cctgtcacat
      601 gataaagatt acaataaggt ccgcctttat gagcacgctg aggcccgcta ttctatgctg
      661 cccagccaag ctaaataagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      721 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgac
      781 gggttttttg tctagagcgc tcaagttgat cgaatgtatg ccccggatat gacctaggga
      841 gattcttcta aagcgagcag ggtttgccaa gtggcgatcg cctcagggaa ccataaccaa
      901 ctttgttcga ggtggcgggg gttgagctgg gaacgatcga gatctagggc gatcgcccgg
      961 agtttttcgg ctttccctac aagaatttcc tggtcaacgt cggtgggttg cctttgggcc
     1021 aaatcgtaca tactaatcgc caggcccgca taaatctccg cctggagggc tggggggaaa
     1081 tcctgggtcg ttgccgccgc taaggcttca taccaacgat cattggcggc ttcgtaatct
     1141 gcattgaggt aatggacaaa acccagggcc atcaaaattt cggggtcgtt gggctgttgg
     1201 gctagggcca tgtcccaatt gtcttgcacc gtggcgatcg ccgttgcttg ggttggcgat
     1261 ggtgggacgg cagccaaagt tgttcagcat gcgccctata gtgagtcgta ttacaattca
     1321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1441 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     1501 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     1561 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     1621 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     1681 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1741 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1801 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1861 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     1921 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1981 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2041 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2101 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2161 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2221 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2281 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2341 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2401 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2461 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2521 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2581 atgtaactcg ccttgatcgt tgggaaccgg agatgaatga agccatacca aacgacgagc
     2641 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2701 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2761 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2821 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2881 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2941 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3001 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3061 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3121 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3181 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3241 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3301 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3361 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3421 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3481 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3541 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3601 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3661 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3721 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3781 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3841 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3901 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3961 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4021 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     4081 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     4141 attacgccaa gctatttagg tgacactata gaatactcaa gctatgaggt gactgccgca
     4201 ctcaatgttc aaccagatct agtgattatt tcggggttag cagccgatgc gggcaacctg
     4261 gtgaagcaac tgcgagaatt aggttacaac ggcattattg ttggggggaa cggcttaaat
     4321 acttctaata ttttccccgt ctgccaagca aaatgtgatg gggtgttggt ggcccaagcc
     4381 tacagtgccg agttagataa tgagattaac cgcgcgtttc gggacgccta ttttcaacaa
     4441 aaccaaaaag agccgcccca atttagtgcc caggctttta cggcgatcca agtttttgtt
     4501 gaagccctca gcagcctcga tgaaaaaacg cccttagaaa ctcttgctct accggacttg
     4561 cgacgacaac tgcgggacga aatttttgca ggtacctacg tcacgccttt gggtgaaatt
     4621 tccttcacag aggaagggga aattgtccag aaggaatttt ttgtggccca aattgaaatg
     4681 gatgaatcgg gtcaacaggg gcgtttcgcc ttcattgaaa cgaacaaagg aattcttaac
     4741 aaaaaagcag gaatatccct atcagtgata gacagacatc cctatcagtg atagatataa
     4801 tgttaactgt gggattgcaa aaggagctcc caactcataa agtcaagtag gagattaatc
     4861 at
//
