LOCUS       sequence-162089-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-Late1 (1wa3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-01 (1wa3)
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(151..180)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(902..921)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(903..921)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agaagacggg tcgttaccga acagagaacg cagagaggtc agcgggtaca gttctttgtc
       61 gatcggctcc tgtttttgcg gtggcgtggt ggtttcaaca ccagaacgga aagattcttc
      121 cggcggtgcg gtcggttccg gacgagactg cagatcttct tcgctaatca gtttctgttc
      181 ggtgcagcca cgaatcttct caacgaacgc cttggccttt tcacgcactt catccggggt
      241 gcctttcact aacgcgctgc ccacgcccac ggccagcacg cccgctttga accattcgca
      301 cacattgtcc agattaacac cgccggttgg cacaaatttc acattcggaa acggaccttt
      361 catcgcttta acaaactgcg gacccaccac ctcacccgga aacagtttta agatggtatg
      421 gcccagtttc atggctttca ccagttcggt cggggtcata acgcctggca tatagaacac
      481 acccttctcc ttgcaaaatt ggctaatttc ctcgtccagg tgcggactca caatgaattc
      541 ggcgccactt tccaccgctt tacggcactg ctccacgctg gtcacggtgc ccgcaccaat
      601 aatcgcgcct ttttccttca gaaagctcag ttccttaatc acggtatccg catccggcac
      661 ggtaaaggta atttcaatca gatgaacgcc gccttcgaaa accgccagcg ctttttcttt
      721 cgcttcttcc acactattgg cacgcagcac ggcaacgatc ttgtgtttct tgaacagctc
      781 ttcgatcttg ctaccagagt cagagccgga gccagattta gaaccggacg cacgcgcacc
      841 catggtacct ccggatcgat aattctagag ggcccgttta aacgctagcc agcttgggtc
      901 tccctatagt gagtcgtatt aatttcgata agccagta
//
