LOCUS       sequence-162107-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recombinant AAV vector packaging self-complementary Renilla 
            Luciferase under the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Paulk NK, Pekrun K, Charville GW, Maguire-Nguyen K, Wosczyna MN, Xu 
            J, Zhang Y, Lisowski L, Yoo B, Vilches-Moure JG, Lee GK, Shrager JB,
            Rando TA, Kay MA
  TITLE     Bioengineered Viral Platform for Intramuscular Passive Vaccine 
            Delivery to Human Skeletal Muscle.
  JOURNAL   Mol Ther Methods Clin Dev. 2018 Jul 24;10:144-155. doi: 
            10.1016/j.omtm.2018.06.001. eCollection 2018 Sep 21.
  PUBMED    30101152
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2018-07-24"; volume: "10:144-155. doi"; 
            pages: " 10.1016/j.omtm.2018.06.001. eCollection 2018 Sep 21"
COMMENT     Sequence Label: pscAAV-CAG-RLuc
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..947
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     primer_bind     1002..1021
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gatccgccac catgacttcg aaagtttatg atccagaaca aaggaaacgg atgataactg
       61 gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt attaattatt
      121 atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac gcggcctctt
      181 cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt attataccag
      241 accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg ttacttgatc
      301 attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag atcatttttg
      361 tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat caagataaga
      421 tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg gatgaatggc
      481 ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa atggttttgg
      541 agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag ttagaaccag
      601 aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt cgtccaacat
      661 tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt gtacaaattg
      721 ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg tttattgaat
      781 cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt cctaatactg
      841 aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat gaaatgggaa
      901 aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc tagagcggcc
      961 gcttcgagca gcctcacctg cgatctcgat gctttatttg tgaaatttgt gatgctattg
     1021 ctttatttgt aaccattata agctgca
//
