LOCUS       sequence-162132-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-4GS-O3-33-myc-Late1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-50
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(811..830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(812..830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(875..928)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(906..926)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtcgttac cgaacagaga acgcagagag gtcagcgggt acagttcttt gtcgatcggc
       61 tcctgttttt gcggtggcgt ggtggtttca acaccagaac ggaaagattc ttccggcggt
      121 gcggtcggtt ccggacgaga ctgcagcagg tcttcttcgg agatcagctt ctggccttcc
      181 accatctgac gccacattgc ttcatgcgga cgcggaatca ggcttgcata caccagcaga
      241 ccgtatgcgc ctgcgctgct gcttgccacg gtcaccgcca gtgccacatc gctcacatcg
      301 cccgccacca ccatataaca tttaccgcca ataccaaatg ccatgtgcac acgcaccagg
      361 gtcacgttgc tacccttcac cgcacgatcc gctgcgctaa tacatgccgc cacgctccag
      421 gtttccacaa tacccaccgc ctgacgctta tccacgctgt tcagaccgct aatcgccggc
      481 agcacgctcg gatgaatgtt tgccagcacc aggctatcca ccagcagttc gcccgcctgg
      541 ctggtaccgg tttcaatcgc ctgctgaatc gcaccaatat caccgcccag catcagcaga
      601 aatttacccg ggctaatggt tttgctcacc agcagatcca cgttcgcgct tttcagcatt
      661 gcatcaccca gttccatacc tgctgcaatg ctggtcagtt ccagaatacc aattgcctgg
      721 ctgctaccgc taccggacgc acgcgcaccc atggtacctc cggatcgata attctagagg
      781 gcccgtttaa acgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa
      841 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag aacctcccaa
      901 ccgtacacgc ctaccgccca tttgcgtcaa tgggggcgga
//
