LOCUS       sequence-162194-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DCAF8, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DCAF8_p.P156S
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1987..2086)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2103..2122)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2104..2122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2127..2143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2127..2143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2574..3365
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3458..4046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3947..3966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4200..4217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4376..4403)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4495..4581)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtccagc aaagggagca gcacagatgg cagaacagac ttagctaatg
      241 gaagcctgtc tagcagtcca gaggagatgt ctggagctga agaggggagg gagacatcct
      301 caggcattga agtggaggcc tcagacctga gtttgagctt gactggggat gatggtggcc
      361 ccaaccgcac cagcacagaa agtcgaggca cagacacaga gagctcaggt gaagataagg
      421 actctgacag catggaggac actggtcatt actccattaa tgatgaaaat cgagtccatg
      481 accgctcaga ggaagaggaa gaggaggaag aagaggagga agaagagcag cctcggcgcc
      541 gtgtacagcg caagcgggct aaccgtgacc aggactcatc agatgatgag cgggccctag
      601 aggactgggt gtcctcagaa acatcagctc taccccgacc tcgctggcaa gccctctctg
      661 cccttcggga gcgggagctg ggttcaagtg cccgctttgt ctatgaggcc tgtggggcaa
      721 gagtctttgt gcagcgtttc cgcctgcagc atgggcttga gggccatact ggttgtgtca
      781 ataccctgca ctttaaccag cgcggcacct ggctggccag tggcagcgat gacctgaagg
      841 tggtggtgtg ggattgggta cggcggcagc cagtactgga ctttgagagt ggccacaaaa
      901 gtaatgtgtt ccaggccaag tttcttccta acagtggtga ttctactctg gccatgtgtg
      961 cccgtgacgg gcaggttcga gtagcagaac tgtctgccac acagtgttgc aagaatacaa
     1021 aacgtgtggc ccagcacaag ggagcgtccc acaagttggc actggaacca gactctccct
     1081 gtacgttctt atctgcaggt gaagatgcag ttgttttcac cattgacctg agacaagacc
     1141 gcccagcgtc gaaactggtg gtgacaaaag agaaagagaa gaaagtgggg ctgtatacga
     1201 tctatgtgaa tcctgccaat acccaccagt ttgcagtggg tggacgagat cagtttgtaa
     1261 ggatttatga ccagaggaaa attgatgaga atgagaacaa tggagtactc aagaagttct
     1321 gtcctcatca cctggtgaac agtgagtcca aagcaaacat cacctgtctt gtgtacagcc
     1381 acgacggcac agagctcctg gccagttaca atgatgaaga catttacctc ttcaactcct
     1441 ctcacagtga tggggcccag tatgttaaga gatacaaggg ccacagaaat aatgccacag
     1501 taaaaggcgt caatttctat ggccccaaga gtgagtttgt ggtgagcggt agtgactgtg
     1561 ggcacatctt cctctgggag aaatcatcct gccagattat tcagttcatg gagggggaca
     1621 agggaggcgt ggtaaactgt cttgagcccc accctcacct gcctgtgctg gcaaccagtg
     1681 gcctagacca tgatgtgaag atctgggcac ccacagctga agcttccact gagctgacag
     1741 ggttaaaaga tgtgattaag aagaacaagc gggagcggga tgaagatagc ttgcaccaaa
     1801 ctgacctgtt tgatagtcac atgctgtggt tccttatgca tcacctgaga cagagacgcc
     1861 atcaccggcg ctggcgagaa cctggggttg gggccacaga cgcggactct gatgagtctc
     1921 ccagctcctc agacacatcg gacgaggagg agggccctga ccgggtgcag tgcatgccat
     1981 cttgattgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2041 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2101 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2161 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2221 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2281 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2341 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2401 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2461 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2521 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2581 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2641 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2701 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2761 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2821 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2881 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2941 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3001 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3061 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3121 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3181 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3241 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3301 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3361 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3421 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3481 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3541 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3601 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3661 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3781 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3841 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3901 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3961 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4021 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4081 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4141 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4201 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4261 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4321 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4381 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4441 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4501 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4561 ctgagccttt cgttttattt gatgcct
//
