LOCUS       sequence-162207-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pentaprobe for assesing the RNA binding potential of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP
  TITLE     A rapid method for assessing the RNA-binding potential of a protein.
  JOURNAL   Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. 
            Epub 2012 Apr 3.
  PUBMED    22492509
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-08"; volume: "40(14):e105. doi"; pages: " 
            10.1093/nar/gks285. Epub 2012 Apr 3"
COMMENT     Sequence Label: Pentaprobe PP5
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(291..309)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(292..309)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(329..346)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    335..559
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(692..711)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     902..923
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggt accgagctcg gatccactag taacggccgc
      121 cagtgtgctg gaattctgca gatcctaatc ccacctagcg tatcgggtca tgtagtgcta
      181 cgttacggcc cccgcccggc atcatattat atcaccccag tgtaatgtgg tgtgaggttg
      241 gagatccatc acactggcgg ccgctcgagc atgcatctag agggccctat tctatagtgt
      301 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      361 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      421 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      481 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      541 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
      601 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      661 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      721 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      781 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
      841 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
      901 agtggactct tgttccaaac tggaacaaca ctc
//
