LOCUS       sequence-162257-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pentaprobe for assesing the RNA binding potential of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP
  TITLE     A rapid method for assessing the RNA-binding potential of a protein.
  JOURNAL   Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. 
            Epub 2012 Apr 3.
  PUBMED    22492509
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-08"; volume: "40(14):e105. doi"; pages: " 
            10.1093/nar/gks285. Epub 2012 Apr 3"
COMMENT     Sequence Label: Pentaprobe PP12
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(131..150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(132..150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(195..398)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(224..244)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        399..778
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctatagaata gggccctcta ggtgaggttg gagtccgacc tggaatctca gcctgacgtg
       61 ccatgcggtg cgatgtcacg ccgcgccacg gtatagtatg gtacgggatc ccggtaccaa
      121 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
      181 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      241 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      301 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      361 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      421 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
      481 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
      541 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
      601 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
      661 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
      721 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
      781 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
      841 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
      901 tcagcgacct ccaa
//
