LOCUS       sequence-162307-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Late1-I3-01-myc-22GS-CaaX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-13
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             892..921
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cgggccctct agaattatcg atccggaggt
      121 accatgctgc agtctcgtcc ggaaccgacc gcaccgccgg aagaatcttt ccgttctggt
      181 gttgaaacca ccacgccacc gcaaaaacag gagccgatcg acaaagaact gtacccgctg
      241 acctctctgc gttctctgtt cggtaacgac ccgtcttctc agaagatcga agagctgttc
      301 aagaaacaca agatcgttgc cgtgctgcgt gccaatagtg tggaagaagc gaaaaagaaa
      361 gcgctggcgg ttttcctggg cggcgttcat ctgattgaaa ttacctttac cgtgccggat
      421 gcggataccg tgattaagga actgagcttt ctgaaggaaa tgggcgcgat tattggtgcg
      481 ggcaccgtga ccagcgtgga gcagtgccgt aaagcggtgg aaagtggcgc cgaattcatt
      541 gtgagtccgc acctggacga ggaaattagc caattttgca aggagaaggg tgtgttctat
      601 atgccaggcg ttatgacccc gaccgaactg gtgaaagcca tgaaactggg ccataccatc
      661 ttaaaactgt ttccgggtga ggtggtgggt ccgcagtttg ttaaagcgat gaaaggtccg
      721 tttccgaatg tgaaatttgt gccaaccggc ggtgttaatc tggacaatgt gtgcgaatgg
      781 ttcaaagcgg gcgtgctggc cgtgggcgtg ggcagcgcgt tagtgaaagg caccccggtg
      841 gaagtggcgg aaaaggccaa ggcgttcgtt gagaagattc gtggctgcac cgaacagaag
      901 ctgatctccg aagaagacct gggcagcggc agtggcgacg gcggtcgtgg tagccgtggc
      961 ggcgatggta gcggcggtag cagcggtaag aagaaaaaga agtcaaagac aaagtgtgta
     1021 attatgtgac tcg
//
