LOCUS       sequence-162390-        4482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM15B, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM15B_WT_V5
FEATURES             Location/Qualifiers
     source          1..4482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1682..1699
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(2361..2460)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2477..2496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2478..2496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2501..2517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2501..2517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2948..3739
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3832..4420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4321..4340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgcccgagg atgaccagcg ggccacgcgc aacctcttca ttggtaacct
      721 ggaccacagc gtatctgagg tggagctgcg aagggccttc gagaaatatg gcatcatcga
      781 ggaggtggtc atcaagaggc ctgcccgtgg ccagggcggt gcctatgcct tcctcaagtt
      841 ccagaacctg gacatggccc atagggctaa ggtggccatg tcgggccgag tgattggtcg
      901 caaccccatt aagataggct atggcaaggc caaccccacc actcgtctct gggtgggtgg
      961 cctgggacct aacacgtcac tggcggctct ggcccgagag tttgaccgct ttgggagcat
     1021 tcggaccatt gatcacgtca aaggagatag ctttgcctat attcagtacg agagcttgga
     1081 cgcagcccag gccgcctgtg ctaaaatgag gggttttccc ttgggtggac cagaccgcag
     1141 gctccgcgtg gattttgcca aagcagagga gactcggtac ccccagcagt accagccctc
     1201 gccactccct gtgcattatg agctgctcac agatggatac acccggcacc gcaacctgga
     1261 cgccgacctg gtgcgggaca ggacgccccc acaccttctg tactcagacc gagaccggac
     1321 ttttttggaa ggggactgga ccagccccag taaaagctct gaccgccgaa acagccttga
     1381 gggctacagt cgctcagtgc gcagccggag tggtgagcgt tggggggcag atggagaccg
     1441 tggtttgccc aagccctggg aagagaagcg gaaacggaga agcctttcca gtgaccgtgg
     1501 gaggacaacc cattcaccat atgaggaacg gagtaggacc aagggcagtg ggcagcagtc
     1561 agagcggggc tccgaccgca cccctgagcg cagccgcaag gagaaccact ccagtgaagg
     1621 gaccaaggag tccagcagca actccctcag caacagcaga catggggctg aggaacgggg
     1681 ccaccaccac caccaccacg aggctgcaga ctcttcccac gggaagaagg caagagacag
     1741 cgagcgcaat caccggacca cagaggccga gcccaagcct ctggaagagc caaaacacga
     1801 gaccaaaaag ctgaagaatc tttcagagta cgctcagaca ctacagctgg gttggaatgg
     1861 gcttctggtg ttgaaaaaca gctgcttccc cacgtctatg catatcctag agggggacca
     1921 gggggtgatc agcagtctcc tcaaagacca cacttctggg agcaagctga cccagctgaa
     1981 gatcgcccag cgccttcgac tggaccagcc caagcttgac gaggtcacac gacgcatcaa
     2041 gcaggggagc cccaacggct atgcggtcct cttagccacc caggcaaccc ccagtgggct
     2101 tggcactgag gggatgccca cagtagagcc cggtctgcag aggcggcttc tcaggaacct
     2161 ggtctcctac ttgaaacaga agcaggccgc aggggtgatc agcttgccag tgggggggtc
     2221 caagggcaga gacggcacag gcatgctcta cgccttccca ccctgcgact tttcccagca
     2281 gtacctccag tcagcactaa ggacattggg caagctagaa gaagaacaca tggtgatagt
     2341 catcgtcaga gacactgcct tgccaacttt cttgtacaaa gttggcatta taagaaagca
     2401 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2461 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2521 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2581 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2641 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2701 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2761 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2821 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2881 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2941 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3001 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3061 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3121 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3181 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3241 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3301 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3361 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3421 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3481 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3541 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3601 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3661 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3721 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3781 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4441 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
