LOCUS       sequence-162405-        4407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PCCB, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_PCCB_WT_V5
FEATURES             Location/Qualifiers
     source          1..4407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     404..421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(580..607)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(699..785)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     834..856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     848..865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     849..865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    881..980
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2598..2697)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2714..2733)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2715..2733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2738..2754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2738..2754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3185..3976
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4069..4407,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      421 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      481 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      541 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
      601 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
      661 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
      721 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
      781 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
      841 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
      901 actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
      961 tttgtacaaa aaagttggca tggcggcggc attacgggtg gcggcggtcg gggcaaggct
     1021 cagcgttctg gcgagcggtc tccgcgccgc ggtccgcagc ctttgcagcc aggccacctc
     1081 tgttaacgaa cgcatcgaaa acaagcgccg gaccgcgctg ctgggagggg gccaacgccg
     1141 tattgacgcg cagcacaagc gaggaaagct aacagccagg gagaggatca gtctcttgct
     1201 ggaccctggc agctttgttg agagcgacat gtttgtggaa cacagatgtg cagattttgg
     1261 aatggctgct gataagaata agtttcctgg agacagcgtg gtcactggac gaggccgaat
     1321 caatggaaga ttggtttatg tcttcagtca ggattttaca gtttttggag gcagtctgtc
     1381 aggagcacat gcccaaaaga tctgcaaaat catggaccag gccataacgg tgggggctcc
     1441 agtgattggg ctgaatgact ctgggggagc acggatccaa gaaggagtgg agtctttggc
     1501 tggctatgca gacatctttc tgaggaatgt tacggcatcc ggagtcatcc ctcagatttc
     1561 tctgatcatg ggcccatgtg ctggtggggc cgtctactcc ccagccctaa cagacttcac
     1621 gttcatggta aaggacacct cctacctgtt catcactggc cctgatgttg tgaagtctgt
     1681 caccaatgag gatgttaccc aggaggagct cggtggtgcc aagacccaca ccaccatgtc
     1741 aggtgtggcc cacagagctt ttgaaaatga tgttgatgcc ttgtgtaatc tccgggattt
     1801 cttcaactac ctgcccctga gcagtcagga cccggctccc gtccgtgagt gccacgatcc
     1861 cagtgaccgt ctggttcctg agcttgacac aattgtccct ttggaatcaa ccaaagccta
     1921 caacatggtg gacatcatac actctgttgt tgatgagcgt gaattttttg agatcatgcc
     1981 caattatgcc aagaacatca ttgttggttt tgcaagaatg aatgggagga ctgttggaat
     2041 tgttggcaac caacctaagg tggcctcagg atgcttggat attaattcat ctgtgaaagg
     2101 ggctcgtttt gtcagattct gtgatgcatt caatattcca ctcatcactt ttgttgatgt
     2161 ccctggcttt ctacctggca cagcacagga atacgggggc atcatccggc atggtgccaa
     2221 gcttctctac gcatttgctg aggcaactgt acccaaagtc acagtcatca ccaggaaggc
     2281 ctatggaggt gcctatgatg tcatgagctc taagcacctt tgtggtgata ccaactatgc
     2341 ctggcccacc gcagagattg cagtcatggg agcaaagggc gctgtggaga tcatcttcaa
     2401 agggcatgag aatgtggaag ctgctcaggc agagtacatc gagaagtttg ccaacccttt
     2461 ccctgcagca gtgcgagggt ttgtggatga catcatccaa ccttcttcca cacgtgcccg
     2521 aatctgctgt gacctggatg tcttggccag caagaaggta caacgtcctt ggagaaaaca
     2581 tgcaaatatt ccattgtacc caactttctt gtacaaagtt ggcattataa gaaagcattg
     2641 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2701 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2761 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2821 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2881 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2941 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     3001 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     3061 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     3121 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     3181 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     3241 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     3301 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     3361 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     3421 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3481 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3541 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3601 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3661 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3721 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3781 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3841 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3901 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3961 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     4021 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4381 gggctgaacg gggggttcgt gcacaca
//
