LOCUS       sequence-162410-        5061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DNTTIP2, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DNTTIP2_WT_V5
FEATURES             Location/Qualifiers
     source          1..5061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     121..137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     121..137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     142..161
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        142..160
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    178..277
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2549..2648)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2664..2681)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2664..2680)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2673..2695)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2744..2830
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2922..2949
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3108..3125)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3279..3867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3359..3378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3960..4751)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggtctagag gaggcatgat
       61 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc
      121 caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatcagc tggatggcaa
      181 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      241 tttcttataa tgccaacttt gtacaagaaa gttggcaaat tgcgaaattt cttcttcttt
      301 cggaactttt ttcctgctgc atttgctgct ttttcagcca tgatctctga gtacttcctt
      361 cggttgtatc ttctgaattc agaatcagcc agcagttctt ccacaatagt tcttttcctt
      421 tgcttcttgg gaattcgtga atggtagaaa tcagctggat tgtcaacaat ggttccaatc
      481 tggaagtact tggggaagcc atctctatca tttttcttgt aaaatctttt cgggtccatg
      541 ctggctctca tcttcagtgc tttgagatca tttttcagtt catttgtcat ttctggagct
      601 ttcataccaa accagccatc ccctgctgtt ttttgtcgtt cttttctgcg ttttttctga
      661 agttgatact ttgattcact atatggtgga acacagtggt ttttttcaaa atcaggtgta
      721 atgacggctt tctgcagaag ctcatttttc tttttctcct tgatctgtgt tagggttctc
      781 ttgttagact gtagtttatc tgcattgaaa ttaatataca aaccacccaa ctgcttgata
      841 ctcagaccag ggtctatgct gctgcttgtc aacttcagaa gtttagcctt tgtgctattt
      901 aggaagtctt cttcatcact aaactcatct tcattttcgt catggtctga tgaatcttct
      961 tcactttttt catcctcttc ctcttctttt tcttcctcaa tggcaacctc acttgccttg
     1021 tcttcctctt ccaagtaaaa atttttatca gcactcattc caggagttgt gtcaattaca
     1081 aacaatgcat tgtcacatga cagacttcct gtgtctccac ttaatgactc cctttggcca
     1141 ctattttcaa caaaacataa agtatcctct tcattctcac tgttttcaga ctgttggctt
     1201 tcatcactgc tgagaactag taagacagaa ttatctttac cctgagatgt gttgggcgca
     1261 gacgtgtata gtttggtatc acattcaaaa tctacattcc cttcactgtt catgtcttca
     1321 ctgacactta taactgtgga ctcttcttca tcatcactac caccacaatc accaaacttt
     1381 gtcaagtcac ttgcttttat ggggctcttt ttgttgttat tccatctgcc tacttccaca
     1441 gttgcaaatg tttgagttaa tgatttcatt acagcctcag agttcagatt agagtgcact
     1501 gatacagcat ttttattttg gggggttgaa tgtctctgag aaactaactg ttgaaggcta
     1561 gtgtcctgaa gttcagaaag attcttcagc tgagaacttt tctcattaat ttctttccca
     1621 tcatctgtta ttccattggc atcttcatcc aaatccttac aattctgttt tgtttcttta
     1681 agagattcaa cattggcctg ttcgtgcact gttaatatat tttctgaact tctgtgggag
     1741 aaatcatcat caaagtcatt attatagaaa tttggcttat ttatctcaga aagagatctt
     1801 gcttgtaaat gggaagtttg tctggtatct gaatcctctg aattcacagg tgtacccacg
     1861 atctgtttct catttcctgg tacaatctta ctatctttct tttcagtttg tgcctttaat
     1921 ttcctctgca tactcctggt tcttctagtt gcaattccag agaatgaaat gtctgagctt
     1981 gatgtctcag catcagatat agcttctgta tgagattctt ggcttggatc tgtcagagat
     2041 ttagccttac ttcttctggc tcctgtagtt ttttctgtgg gaagcacaat tctagaaata
     2101 cctgaaacat gagattctgc ttcagacact atttcttcag tgtaagactc ctttgttgga
     2161 gttactttcg gctttttcct aacactggac actggggagc atgcaattaa gatctgcctt
     2221 ctcctagtta cccttaaaat ggtatcatgg tgctcagaca cagaataatt tgactctgcc
     2281 tcagaggtct ctccatccgt agatggttca gtcccctttg gtagtgagcc tgtagttctg
     2341 ctcttcctct ttctagcttt aggagttcta gggattaaac tttgcttccc agtggtctgt
     2401 gattcagcag tagttcgggc atcagatcca gtactacttt ctggatgcgc ttgaatccca
     2461 ttagcagcaa aactcttttg cccggaactt tcagccgacg cggcttggat gctggcctta
     2521 gcccgtgcag atctggtaac caccatggtg ccaacttttt tgtacaaagt tggcattata
     2581 aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     2641 attatttggg gcccgagctt aagactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2701 atccatgcta gcgttaacgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     2761 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     2821 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
     2881 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
     2941 tggccttttt gcgtttctac aaactcttcc tggctagcgg tacgcgtatt aattgcgttg
     3001 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3061 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3901 gctcagtgga acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgagggt
     3961 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     4021 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     4081 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     4141 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     4201 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     4261 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     4321 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     4381 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     4441 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     4501 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     4561 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     4621 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     4681 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     4741 gcttccctca taatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     4801 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     4861 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     4921 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     4981 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
     5041 cacggtgtgc gtcacccggc a
//
