LOCUS       sequence-162420-        3450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RIT1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RIT1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(37..625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(117..136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(718..1509)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     1940..1956
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1940..1956
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1961..1980
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1961..1979
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1997..2096
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2757..2856)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2872..2889)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2872..2888)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2881..2903)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2952..3038
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3130..3157
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3316..3333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
       61 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      661 tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc tcgagggtta
      721 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
      781 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
      841 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
      901 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
      961 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     1021 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     1081 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     1141 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     1201 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
     1261 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
     1321 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
     1381 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
     1441 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
     1501 ttccctcata atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
     1561 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     1621 catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1681 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1741 ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca
     1801 cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct
     1861 ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
     1921 agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact cactataggg
     1981 gatatcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
     2041 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ttggcaaagt
     2101 tactgaatct ttcttcttcc ggaatggtga ttttagcctc ttccatacac tgtttttggg
     2161 cttagatttt ttctccatgg ccagtactgc ctccttttct ttcctacgta tctcccgtac
     2221 aagggcatgg aaaacatcat caatatagta gcggtatgca gcagatgtct caaaaaaggg
     2281 acagctgaat tctcgggcca aggccaatcc ttcttccttg gtgacctgtc ttagctgttt
     2341 gaggtctgac ttgtttccca caagaaccac aggtgtatcg tcagtacgtc ggactcgata
     2401 aataagctgt ttaaactcac gaacttcatg gaaacttcga cgatccgtga tagagtaaca
     2461 gatgataaac ccttctcctg ccctcatata ctggtcccgc atggctgtaa actctgcctg
     2521 tccagctgta tccaaaatgt ccagattggc aggctcatca tcaatacgga tcctgatctt
     2581 ataagcatct tcaatggtgg gatcatgatc ttctgggaat cggtggctga tgaactgcat
     2641 ggtcatggca ctcttcccta caccaccagc acccagcatc actagtttgt actcccgtga
     2701 gagcccagcg gggctgctac agcagctacc aactgggcga gttccagaat ccatggtgcc
     2761 aacttttttg tacaaagttg gcattataaa aaagcattgc tcatcaattt gttgcaacga
     2821 acaggtcact atcagtcaaa ataaaatcat tatttggggc ccgagcttaa gactggccgt
     2881 cgttttacaa cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa
     2941 ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct
     3001 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3061 ttgtgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     3121 aaactaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg
     3181 gctagcggta cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3241 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3301 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3421 aaagaacatg tgagcaaaag gccagcaaaa
//
