LOCUS       sequence-162429-        9531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses huLbCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY027
FEATURES             Location/Qualifiers
     source          1..9531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        170..418
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     170..190
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     341..360
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        539..918
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        919..1122
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1073..1093
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1167..1186
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1167..1185
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1233..4916
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4917..4964
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4971..5051
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5125..5142)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5131..5355
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5401..5829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5488..5507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5698..5719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5838..5858)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5843..6172
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6023..6158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6085..6104
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6239..7033
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6293..6312)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6903..6922
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7207..7328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7244..7263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7298..7317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7377..7393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7377..7393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7390..7412)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7401..7417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7425..7431,7432..7449,7450..7455))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7470..7491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7608..7625)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7779..8364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7859..7878)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8535..9326,9327..9395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9158..9177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9396..9500)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga cttaattaag agggcctatt
      181 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      241 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      301 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      361 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgt
      421 aatttctact aagtgtagat ggagacgata tatcgtctct ttttcaattg catgaagaat
      481 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      541 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      601 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      661 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      721 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      781 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      841 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      901 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      961 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1021 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1081 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     1141 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
     1201 tagcgtttaa acttaagctt ggtaccgcca ccatgagcaa gctggagaag tttacaaact
     1261 gctactccct gtctaagacc ctgaggttca aggccatccc tgtgggcaag acccaggaga
     1321 acatcgacaa taagcggctg ctggtggagg acgagaagag agccgaggat tataagggcg
     1381 tgaagaagct gctggatcgc tactatctgt cttttatcaa cgacgtgctg cacagcatca
     1441 agctgaagaa tctgaacaat tacatcagcc tgttccggaa gaaaaccaga accgagaagg
     1501 agaataagga gctggagaac ctggagatca atctgcggaa ggagatcgcc aaggccttca
     1561 agggcaacga gggctacaag tccctgttta agaaggatat catcgagaca atcctgccag
     1621 agttcctgga cgataaggac gagatcgccc tggtgaacag cttcaatggc tttaccacag
     1681 ccttcaccgg cttctttgat aacagagaga atatgttttc cgaggaggcc aagagcacat
     1741 ccatcgcctt caggtgtatc aacgagaatc tgacccgcta catctctaat atggacatct
     1801 tcgagaaggt ggacgccatc tttgataagc acgaggtgca ggagatcaag gagaagatcc
     1861 tgaacagcga ctatgatgtg gaggatttct ttgagggcga gttctttaac tttgtgctga
     1921 cacaggaggg catcgacgtg tataacgcca tcatcggcgg cttcgtgacc gagagcggcg
     1981 agaagatcaa gggcctgaac gagtacatca acctgtataa tcagaaaacc aagcagaagc
     2041 tgcctaagtt taagccactg tataagcagg tgctgagcga tcgggagtct ctgagcttct
     2101 acggcgaggg ctatacatcc gatgaggagg tgctggaggt gtttagaaac accctgaaca
     2161 agaacagcga gatcttcagc tccatcaaga agctggagaa gctgttcaag aattttgacg
     2221 agtactctag cgccggcatc tttgtgaaga acggccccgc catcagcaca atctccaagg
     2281 atatcttcgg cgagtggaac gtgatccggg acaagtggaa tgccgagtat gacgatatcc
     2341 acctgaagaa gaaggccgtg gtgaccgaga agtacgagga cgatcggaga aagtccttca
     2401 agaagatcgg ctccttttct ctggagcagc tgcaggagta cgccgacgcc gatctgtctg
     2461 tggtggagaa gctgaaggag atcatcatcc agaaggtgga tgagatctac aaggtgtatg
     2521 gctcctctga gaagctgttc gacgccgatt ttgtgctgga gaagagcctg aagaagaacg
     2581 acgccgtggt ggccatcatg aaggacctgc tggattctgt gaagagcttc gagaattaca
     2641 tcaaggcctt ctttggcgag ggcaaggaga caaacaggga cgagtccttc tatggcgatt
     2701 ttgtgctggc ctacgacatc ctgctgaagg tggaccacat ctacgatgcc atccgcaatt
     2761 atgtgaccca gaagccctac tctaaggata agttcaagct gtattttcag aaccctcagt
     2821 tcatgggcgg ctgggacaag gataaggaga cagactatcg ggccaccatc ctgagatacg
     2881 gctccaagta ctatctggcc atcatggata agaagtacgc caagtgcctg cagaagatcg
     2941 acaaggacga tgtgaacggc aattacgaga agatcaacta taagctgctg cccggcccta
     3001 ataagatgct gccaaaggtg ttcttttcta agaagtggat ggcctactat aaccccagcg
     3061 aggacatcca gaagatctac aagaatggca cattcaagaa gggcgatatg tttaacctga
     3121 atgactgtca caagctgatc gacttcttta aggatagcat ctcccggtat ccaaagtggt
     3181 ccaatgccta cgatttcaac ttttctgaga cagagaagta taaggacatc gccggctttt
     3241 acagagaggt ggaggagcag ggctataagg tgagcttcga gtctgccagc aagaaggagg
     3301 tggataagct ggtggaggag ggcaagctgt atatgttcca gatctataac aaggactttt
     3361 ccgataagtc tcacggcaca cccaatctgc acaccatgta cttcaagctg ctgtttgacg
     3421 agaacaatca cggacagatc aggctgagcg gaggagcaga gctgttcatg aggcgcgcct
     3481 ccctgaagaa ggaggagctg gtggtgcacc cagccaactc ccctatcgcc aacaagaatc
     3541 cagataatcc caagaaaacc acaaccctgt cctacgacgt gtataaggat aagaggtttt
     3601 ctgaggacca gtacgagctg cacatcccaa tcgccatcaa taagtgcccc aagaacatct
     3661 tcaagatcaa tacagaggtg cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg
     3721 gcatcgatag gggcgagcgc aatctgctgt atatcgtggt ggtggacggc aagggcaaca
     3781 tcgtggagca gtattccctg aacgagatca tcaacaactt caacggcatc aggatcaaga
     3841 cagattacca ctctctgctg gacaagaagg agaaggagag gttcgaggcc cgccagaact
     3901 ggacctccat cgagaatatc aaggagctga aggccggcta tatctctcag gtggtgcaca
     3961 agatctgcga gctggtggag aagtacgatg ccgtgatcgc cctggaggac ctgaactctg
     4021 gctttaagaa tagccgcgtg aaggtggaga agcaggtgta tcagaagttc gagaagatgc
     4081 tgatcgataa gctgaactac atggtggaca agaagtctaa tccttgtgca acaggcggcg
     4141 ccctgaaggg ctatcagatc accaataagt tcgagagctt taagtccatg tctacccaga
     4201 acggcttcat cttttacatc cctgcctggc tgacatccaa gatcgatcca tctaccggct
     4261 ttgtgaacct gctgaaaacc aagtatacca gcatcgccga ttccaagaag ttcatcagct
     4321 cctttgacag gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc ctggactata
     4381 agaacttctc tcgcacagac gccgattaca tcaagaagtg gaagctgtac tcctacggca
     4441 accggatcag aatcttccgg aatcctaaga agaacaacgt gttcgactgg gaggaggtgt
     4501 gcctgaccag cgcctataag gagctgttca acaagtacgg catcaattat cagcagggcg
     4561 atatcagagc cctgctgtgc gagcagtccg acaaggcctt ctactctagc tttatggccc
     4621 tgatgagcct gatgctgcag atgcggaaca gcatcacagg ccgcaccgac gtggattttc
     4681 tgatcagccc tgtgaagaac tccgacggca tcttctacga tagccggaac tatgaggccc
     4741 aggagaatgc catcctgcca aagaacgccg acgccaatgg cgcctataac atcgccagaa
     4801 aggtgctgtg ggccatcggc cagttcaaga aggccgagga cgagaagctg gataaggtga
     4861 agatcgccat ctctaacaag gagtggctgg agtacgccca gaccagcgtg aagcacaaaa
     4921 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc tacccatacg
     4981 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
     5041 ccgactatgc ctaagaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag
     5101 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5161 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5221 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5281 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5341 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     5401 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5461 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5521 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5581 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5641 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5701 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5761 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5821 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6001 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6061 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6121 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6181 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     6241 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6301 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6361 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6421 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6481 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6541 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6601 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6661 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6721 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6781 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6841 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6901 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6961 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7021 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7081 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7141 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7201 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7261 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7321 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7381 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7441 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7501 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7561 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7621 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7681 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7741 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7801 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7861 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7921 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7981 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8041 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8101 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8161 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8221 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8281 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt
     8341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8461 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8521 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8581 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8641 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8701 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8761 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8821 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8881 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8941 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9001 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9061 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9121 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9181 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9241 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9301 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9361 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9421 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9481 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
